Metabotropic glutamate receptors potentiate responses to NMDA and AMPA from layer V cells in rat visual cortex.
Metabotropic glutamate receptors potentiate responses to NMDA and AMPA from layer V cells in rat visual cortex.
复制标题
代谢型谷氨酸受体增强大鼠视觉皮层 V 层细胞对 NMDA 和 AMPA 的反应。
DOI：
10.1152/jn.1996.76.2.808
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Daw,NW
中科院分区：
文献类型：
--
作者：
Wang,XF;Daw,NW
1. 利用大鼠视觉皮层切片V层细胞的全细胞记录，我们证明了代谢性谷氨酸激动剂(1S,3R)-1-氨基-1,3-环戊二羧酸对n -甲基- d -天冬氨酸（NMDA）的反应增强。这种增强在几秒钟内发生并持续几分钟。2. 灌注液中可见河豚毒素增强，但移液管溶液中的鸟苷5′- o -（2-硫代二磷酸）消除了增强，表明这是突触后现象。这种增强作用也被浴液中的α -甲基-4-羧基苯基甘氨酸所消除，证实它是由于代谢性谷氨酸受体引起的。3. 在31例试验中，29例对α -氨基-3-羟基-5-甲基-4-异恶唑烯丙酸的反应也增强了，并且在NMDA拮抗剂d -2-氨基-5-膦酸盐存在时，这种增强仍然存在。
1. With the use of whole cell recordings of layer V cells from slices of rat visual cortex, we demonstrate a potentiation of the response to N-methyl-D-aspartate (NMDA) by the metabotropic glutamate agonist (1S,3R)-1-Amino-1,3-cyclopentanedicarboxylic acid. This potentiation occurs within a few seconds and lasts a few minutes. 2. The potentiation is seen with tetrodotoxin in the perfusion solution, but is abolished by guanosine 5'-O-(2-thiodiphosphate) in the pipette solution, showing that it is a postsynaptic phenomenon. The potentiation is also abolished by alpha-methyl-4-carboxyphenylglycine in the bath solution, confirming that it is due to metabotropic glutamate receptors. 3. In 29 of 31 cases tested, the response to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid was also potentiated, and the potentiation remained in the presence of the NMDA antagonist D-2-amino-5-phosphonovalerate.