Regulation of Cell Death and Innate Immunity by Two Receptor-like Kinases in Arabidopsis
Regulation of Cell Death and Innate Immunity by Two Receptor-like Kinases in Arabidopsis
复制标题
DOI：
10.1016/j.chom.2009.05.019
复制
发表时间：
2009-07-23
影响因子：
30.3
通讯作者：
Zhang, Yuelin
中科院分区：
文献类型：
--
作者：
Gao, Minghui;Wang, Xia;Zhang, Yuelin
在识别细菌鞭毛蛋白后，植物受体FLS 2与油菜素类固醇不敏感1相关受体激酶1（BAK 1）异二聚化并激活植物防御反应。由于防御反应的组成性激活是有害的，植物抗性信号传导途径必须受到负控制，尽管所涉及的机制尚不清楚。我们确定拟南芥BIR 1作为BAK 1相互作用的受体样激酶。敲除BIR 1导致广泛的细胞死亡、组成性防御反应的激活以及鞭毛蛋白对MPK 4（植物抗性（R）蛋白信号传导的负调节物）的激活的损害。在筛选BIR 1 -1表型的抑制子中获得的突变体SOBIR 1 -1挽救了在BIR 1 -1中观察到的细胞死亡。SOBIR 1编码另一种受体样激酶，其过表达激活细胞死亡和防御反应。我们的数据表明，BIR 1负调控多种植物抗性信号传导途径，其中之一是SOBIR 1依赖的途径在这里确定。
Upon recognition of bacterial flagellin, the plant receptor FLS2 heterodimerizes with brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) and activates plant defense responses. Because constitutive activation of defense responses is detrimental, plant resistance signaling pathways must be negatively controlled, although the mechanisms involved are unclear. We identified Arabidopsis BIR1 as a BAK1-interacting receptor-like kinase. Knocking out BIR1 leads to extensive cell death, activation of constitutive defense responses, and impairment in the activation of MPK4, a negative regulator of plant resistance (R) protein signaling, by flagellin. sobir1-1, a mutant obtained in a screen for suppressors of the bir1-1 phenotype, rescued cell death observed in bir1-1. SOBIR1 encodes another receptor-like kinase whose overexpression activates cell death and defense responses. Our data suggest that BIR1 negatively regulates multiple plant resistance signaling pathways, one of which is the SOBIR1-dependent pathway identified here.