Avoidance of graft versus host disease by proper titration of cell and radiation dose in a mouse to rat xeno-chimera.
Avoidance of graft versus host disease by proper titration of cell and radiation dose in a mouse to rat xeno-chimera.
复制标题
通过适当滴定小鼠与大鼠异种嵌合体中的细胞和辐射剂量，避免移植物抗宿主病。
DOI：
10.1016/s0041-1345(00)01050-2
复制
发表时间：
2000
影响因子：
0.9
通讯作者：
DiSesa,VJ
中科院分区：
文献类型：
--
作者：
Mohiuddin,MM;Qian,X;Qin,Y;DiSesa,VJ
方法从B10中制备250至4亿个骨髓细胞。 BR 小鼠（Jackson Laboratory，Bar Harbor，Maine）被注射到经致死辐射（950 至 1100 cGy）的 Lewis 大鼠（Harlan Sprague Dawley，Indianapolis，Ind）中。使用抗大鼠 (RT1Aavl-FITC) 和抗小鼠 (H2Kk-PE) 单克隆抗体 (Pharmingen, San Diego, CA) 通过流式细胞术分析对存活 30 天的动物的外周血淋巴细胞进行分型，以确定其相对百分比。监测所有动物的 GVHD 迹象。严重腹泻、脱发、皮炎和消瘦是 GVHD 的发病标志。在可疑大鼠中寻找 GVHD 的组织学证据。
METHODSTwo hundred fifty to four hundred million bone marrow cells were prepared from B10. BR mice (Jackson Laboratory, Bar Harbor, Maine) and were injected into lethally irradiated (950 to 1100 cGy) Lewis rats (Harlan Sprague Dawley, Indianapolis, Ind). Peripheral blood lymphocytes of animals surviving for 30 days were typed by ffow cytometric analysis using anti-rat (RT1Aavl-FITC) and antimouse (H2Kk-PE) monoclonal antibodies (Pharmingen, San Diego, Calif) to determine their relative percentages. All animals were monitored for signs of GVHD. Severe diarrhea, loss of hair, dermatitis, and wasting mark the onset of GVHD. In suspect rats histologic evidence of GVHD was sought.