A Simple Microfluidic Electrochemical HPLC Detector for Quantifying Fenton Reactivity from Welding Fumes.
A Simple Microfluidic Electrochemical HPLC Detector for Quantifying Fenton Reactivity from Welding Fumes.
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DOI：
10.1039/c4ay01534g
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发表时间：
2014-10-21
期刊：
影响因子：
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通讯作者：
Henry CS
中科院分区：
文献类型：
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作者：
Pluangklang T;Wydallis JB;Cate DM;Nacapricha D;Henry CS
描述了一种简单的微流体电化学流动电池的开发和表征，该电池可以与高效液相色谱相结合，以实现双吸光度/电化学检测。耦合吸收和电化学检测增加了从单次注入中收集的信息，但需要第二个（通常昂贵的）检测系统。在这里，一个廉价的，可定制的微流体电化学检测器与商业HPLC/UV系统串联在一起。该微流控装置由聚二甲基硅氧烷制成，并含有碳糊电极。为验证该双检测系统的实用性，对Fenton化学生成的自由基清除剂水杨酸和羟基自由基的反应产物进行了分析。采用双检测系统对加入H2O2过滤焊接烟尘样品产生的2,5-二羟基苯甲酸、2,3-二羟基苯甲酸和儿茶酚进行定量。测定回收率高，对照样品中2,5-二羟基苯甲酸、2,3-二羟基苯甲酸和儿茶酚的回收率分别为97-102%、92-103%和95-103%。该方法简单、可靠，可将电化学检测与HPLC-UV系统相结合，成本低廉。
Development and characterization of a simple microfluidic electrochemical flow cell that can be coupled with HPLC to enable dual absorbance/electrochemical detection is described. Coupling absorbance and electrochemical detection increases the information that can be gathered from a single injection, but a second (typically expensive) detection system is required. Here, an inexpensive, customizable microfluidic electrochemical detector is coupled in series with a commercial HPLC/UV system. The microfluidic device is made from poly(dimethylsiloxane) and contains carbon paste electrodes. To demonstrate the utility of this dual-detection system, the reaction products of the radical scavenging agent salicylic acid and hydroxyl radical generated by Fenton chemistry were analyzed. The dual-detection system was used to quantify 2,5-dihydroxybenzoic acid, 2,3-dihydroxybenzoic acid, and catechol produced by the addition of H2O2 to filter samples of welding fumes. Measurement recovery was high, with percent recoveries between 97-102%, 92-103%, and 95-103% for 2,5-dihydroxybenzoic acid, 2,3-dihydroxybenzoic acid, and catechol, respectively, for control samples. The methods described in this work are simple, reliable, and can inexpensively couple electrochemical detection to HPLC-UV systems.