TRANSFORMATION BY POLYOMA VIRUS AFFECTS ADHESION OF FIBROBLASTS
TRANSFORMATION BY POLYOMA VIRUS AFFECTS ADHESION OF FIBROBLASTS
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DOI：
10.1038/newbio231147a0
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
WILLIAMS, JF
中科院分区：
文献类型：
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作者：
EDWARDS, JG;CAMPBELL, JA;WILLIAMS, JF
当新鲜胰蛋白酶处理的仓鼠成纤维细胞（BHK 21，克隆 131）在悬浮液中摇动时，细胞间粘附迅速形成，并且有一些支持这一观点，即该过程是培养物中细胞粘附特性的表达，该细胞在用于分离它们的程序中幸存下来，而不仅仅是使用胰蛋白酶2的人为因素。正如各种技术所显示的那样，肿瘤病毒对培养物中的动物细胞的转化会改变细胞表面3-9，并且我们研究了由我们一直使用的特定测定所定义的细胞间粘附性是否也受到影响。
WHEN freshly trypsinized hamster fibroblasts (BHK 21, Clone 131) are shaken in suspension, intercellular adhesions are rapidly formed and there is some support for the view that this process is the expression of an adhesive property of the cells in culture which has survived the procedures used to separate them and is not simply an artefact of the use of trypsin2. Transformation of animal cells in culture by tumour viruses alters the cell surfaces, as various techniques have shown3–9, and we have investigated whether intercellular adhesiveness, as defined by the particular assay we have been using, is also affected.