Two ancestral APETALA3 homologs from the basal angiosperm Magnolia wufengensis (Magnoliaceae) can affect flower development of Arabidopsis
Two ancestral APETALA3 homologs from the basal angiosperm Magnolia wufengensis (Magnoliaceae) can affect flower development of Arabidopsis
复制标题
来自基部被子植物五峰木兰（木兰科）的两个祖先 APETALA3 同源物可以影响拟南芥花的发育
DOI：
10.1016/j.gene.2013.11.076
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发表时间：
2014-03-01
期刊：
影响因子：
3.5
通讯作者：
Chen, Faju
中科院分区：
文献类型：
--
作者：
Jing, Danlong;Liu, Zhixiong;Chen, Faju
APETALA3(AP3)同源物参与了在核心优树模式生物中确定花瓣和雄蕊的身份。为了研究AP3同源物在核心被子植物和基生被子植物之间的功能保守性，我们从木本植物五峰木本植物中分离和鉴定了两个AP3同源物。序列和系统发育分析表明，这两个基因都是古AP3谱系的分支成员。此外，在MAwuAP3_1/2蛋白的C-末端区域发现了一个高度保守的古AP3基序，但通常存在于AP3/DEF样谱系成员中的PI衍生基序缺失。Send-Quantity和Real-Time PCR分析表明，MAwuAP3_1/2基因仅在花被和雄蕊中表达。然而，MAwuAP3_1在花被和雄蕊迅速增大的过程中保持较高的表达水平，而MAwuAP3_2的表达仅在花被和雄蕊发育的早期检测到。此外，MAwuAP3_1/2在转基因拟南芥中的表达引起的表型变化与内源AP3基因的异源表达所引起的表型变化部分相似。此外，35S：：MAwuAP3_1/2转基因拟南芥可部分用于挽救功能丧失的拟南芥AP3突变体(AP3-3)。这些发现需要更全面地理解从基生被子植物到核心真胚轴分支的B-功能进化。(C)2013爱思唯尔B.V.保留所有权利。
APETALA3 (AP3) homologs are involved in specifying petal and stamen identities in core eudicot model organisms. In order to investigate the functional conservation of AP3 homologs between core eudicots and basal angiosperm, we isolated and identified two AP3 homologs from Magnolia wufengensis, a woody basal angiosperm belonging to the family Magnoliaceae. Sequence and phylogenetic analyses revealed that both genes are clade members of the paleoAP3 lineage. Moreover, a highly conserved motif of paleoAP3 is found in the C-terminal regions of MAwuAP3_1/2 proteins, but the PI-derived motif, usually present in AP3/DEF-like lineage members, is missing. Send-quantitative and real time PCR analyses showed that the expression of MAwuAP3_1/2 was restricted to tepals and stamens. However, the MAwuAP3_1 expression was maintained at a high level during the rapid increased in size of tepals and stamens, while MAwuAP3_2 mRNA was only detected at the early stage of tepal and stamen development. Furthermore, the expression of MAwuAP3_1/2 in transgenic Arabidopsis causes phenotypic changes which partly resemble those caused by ectopic expressions of the endogenous AP3 gene. Moreover, the 35S::MAwuAP3_1/2 transgenic Arabidopsis can be used partially to rescue the loss-of-function ap3 mutant (ap3-3) of Arabidopsis. These findings call for a more comprehensive understanding of the B-functional evolution from basal angiosperm to core eudicot clades. (C) 2013 Elsevier B.V. All rights reserved.