Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants
Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants
复制标题
DOI：
10.1039/c0lc00135j
复制
发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Faris, Ronald A.
中科院分区：
文献类型：
--
作者：
Goral, Vasiliy N.;Hsieh, Yi-Cheng;Faris, Ronald A.
我们描述了一种用于三维（3D）动态原代人肝细胞培养的基于灌注的微流体装置。该微流控装置在体外不添加生物或合成基质或凝血剂的情况下，通过恢复肝细胞的膜极性和转运功能，促进和维持原代人肝细胞的三维组织样细胞形态和细胞特异性功能。我们的动态细胞培养装置的一个独特之处在于创造了一个微环境，不添加生物或合成基质或凝血剂，促进肝细胞的三维组织形成索状结构，表现出功能性膜极性，这一点可以通过间隙连接的表达和扩展的、功能活跃的胆管网络的形成来证明。
We describe a perfusion-based microfluidic device for three-dimensional (3D) dynamic primary human hepatocyte cell culture. The microfluidic device was used to promote and maintain 3D tissue-like cellular morphology and cell-specific functionality of primary human hepatocytes by restoring membrane polarity and hepatocyte transport function in vitro without the addition of biological or synthetic matrices or coagulants. A unique feature of our dynamic cell culture device is the creation of a microenvironment, without the addition of biological or synthetic matrices or coagulants, that promotes the 3D organization of hepatocytes into cord-like structures that exhibit functional membrane polarity as evidenced by the expression of gap junctions and the formation of an extended, functionally active, bile canalicular network.