Soil microbial communities associated with Bt and non-Bt corn in three soils
Soil microbial communities associated with Bt and non-Bt corn in three soils
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DOI：
10.2134/jeq2004.0832
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发表时间：
2004-05-01
影响因子：
2.4
通讯作者：
Buyer, JS
中科院分区：
文献类型：
--
作者：
Blackwood, CB;Buyer, JS
在生长室实验中评估了Bt玉米（经工程改造以表达苏云金芽孢杆菌毒素的转基因玉米）表达Cry内毒素对土壤微生物群落结构的影响。在具有不同质地的三种土壤类型中，将表达不同Cry内毒素的两个转基因玉米品系与它们各自的非转基因株系进行比较。利用非整土磷脂脂肪酸（PLFA）剖面和根际群落水平生理剖面（CLPP）对群落结构进行了评价。由于土壤类型的PLFA配置文件的差异是显着的，占73%的数据集的总变异。由于土壤类型的细菌和真菌CLPP配置文件的差异有统计学意义，但可能不是生物学上的重要性，占6.3和3.8%的总变异，分别。Cry内毒素和玉米系的表达对微生物谱都没有显著影响，除了在高粘土土壤中，这两个因素都显著影响细菌CLPP谱（分别占该土壤变异性的6.6%和6.1%）。Cry内毒素的表达也显著降低了散装土壤中真核PLFA生物标志物的存在，尽管尚不清楚哪些真核生物群受到影响。我们的结论是，转基因Bt玉米在这个短期实验的影响是小的，长期的调查是必要的。
The effects of expression of Cry endotoxin by Bt corn (transgenic corn engineered to express Bacillus thuringiensis toxin) on soil microbial community structure were assessed in a growth chamber experiment. Two lines of transgenic corn expressing different Cry endotoxins were compared with their respective non-transgenic isolines in three soil types with differing textures. Phospholipid fatty acid (PLFA) profiles from bulk soil and community-level physiological profiles (CLPP) from the rhizosphere community were used to assess community structure. Differences in PLFA profiles due to soil type were significant, accounting for 73% of the total variability in the dataset. Differences in bacterial and fungal CLPP profiles due to soil type were statistically significant, but probably not biologically important, accounting for 6.3 and 3.8% of the total variability, respectively. Neither expression of Cry endotoxin nor corn line had a significant effect on microbial profiles, except in the high-clay soil where both factors significantly affected bacterial CLPP profiles (accounting for 6.6 and 6.1% of the variability in that soil, respectively). Expression of Cry endotoxin also significantly reduced the presence of eukaryotic PLFA biomarker in bulk soils, although it is unclear which groups of eukaryotes were affected. We conclude that the effects of transgenic Bt corn in this short-term experiment are small, and longer-term investigations are necessary.