CD40 expression by human monocytes: regulation by cytokines and activation of monocytes by the ligand for CD40.
CD40 expression by human monocytes: regulation by cytokines and activation of monocytes by the ligand for CD40.
复制标题
DOI：
10.1084/jem.178.2.669
复制
发表时间：
1993-08-01
影响因子：
15.3
通讯作者：
Spriggs, M K
中科院分区：
文献类型：
--
作者：
Alderson, M R;Armitage, R J;Tough, T W;Strockbine, L;Fanslow, W C;Spriggs, M K
CD 40是细胞表面蛋白质的肿瘤坏死因子（TNF）受体家族的成员，并且最初被描述为B细胞限制性抗原。用粒细胞/巨噬细胞集落刺激因子（GM-CSF）、白细胞介素3（IL-3）或干扰素γ（IFN-γ）处理原代人单核细胞导致CD 40 mRNA的诱导和细胞表面蛋白表达的增强。发现CD 40介导单核细胞与表达重组CD 40配体的细胞的粘附。CD 40配体转染的细胞在GM-CSF、IL-3或IFN-γ存在下为单核细胞TNF-α和IL-6的产生提供了有效的共刺激，并增强了GM-CSF或IL- 3刺激的IL-8的产生。此外，CD 40配体转染的细胞在不存在共刺激物的情况下作用，诱导单核细胞对人黑素瘤细胞靶点具有杀肿瘤作用。总的来说，这些数据表明，CD 40配体是多效性的，对单核细胞具有有效的生物活性。
CD40 is a member of the tumor necrosis factor (TNF) receptor family of cell surface proteins and was originally described as a B cell restricted antigen. Treatment of primary human monocytes with granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin 3 (IL-3), or interferon gamma (IFN-gamma) resulted in the induction of CD40 mRNA and enhancement of cell surface protein expression. CD40 was found to mediate monocyte adhesion to cells expressing recombinant CD40 ligand. CD40 ligand-transfected cells provided a potent costimulus for monocyte TNF-alpha and IL-6 production in the presence of GM-CSF, IL-3, or IFN-gamma, and enhanced IL-8 production stimulated by GM-CSF or IL- 3. In addition, CD40 ligand-transfected cells acting in the absence of a costimulus induced monocytes to become tumoricidal against a human melanoma cell target. Collectively, these data indicate that CD40 ligand is pleiotropic with potent biological activity on monocytes.