REACTIONS OF GLYOXAL WITH NUCLEIC ACIDS NUCLEOTIDES AND THEIR COMPONENT BASES
REACTIONS OF GLYOXAL WITH NUCLEIC ACIDS NUCLEOTIDES AND THEIR COMPONENT BASES
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DOI：
10.1016/0005-2787(68)90290-6
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发表时间：
1968-01-01
期刊：
影响因子：
--
通讯作者：
SHIBATA, K
中科院分区：
文献类型：
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作者：
NAKAYA, K;TAKENAKA, O;SHIBATA, K
通过测量光谱变化来检查乙二醛与核酸、核苷酸及其碱基的反应。经过高浓度乙二醛处理后，RNA 和 DNA 中所有碱基和核苷酸的光谱均发生明显变化。然而，当用低浓度的乙二醛处理时，只有鸟嘌呤或鸟苷酸发生特定的光谱变化。乙二醛与该碱基或其核苷酸的反应在30分钟内完成。根据室温下的光谱变化估计，pH 值在 8.3 至 9.8 之间。利用这种现象来测定核酸中的鸟苷含量，结果与以前通过色谱法获得的数据非常吻合。从光谱变化判断，乙二醛不与天然小牛胸腺DNA发生反应，而与热变性DNA发生显着反应。用变性DNA获得的光谱变化是2个性质不同的变化的组合，该变化仅归因于乙二醛和鸟苷残基之间的反应以及260-mμ带的增色位移，这可能是反应的次级效应。根据对这两种效应的分析，估计变性DNA中鸟苷残基的反应度为72°/o。
The reactions of glyoxal with nucleic acids, nucleotides and their bases were examined by measuring spectral changes. The spectra of all of the bases and nucleotides in RNA and DNA were changed appreciably by treatment with a high concentration of glyoxal. When treated with a low concentration of glyoxal, however, only guanine or guanylic acid underwent a specific spectral change. The reaction of glyoxal with this base or its nucleotide proceeded to completion within 30 min. between pH 8.3 and 9.8 as estimated from the spectral change at room temperature. This phenomenon was used to determine the guanosine content of nucleic acids and the results agreed well with previous data obtained by chromatog-raphy. As judged from the spectral change, glyoxal did not react with native calf thymus DNA, while it reacted considerably with heat-denatured DNA. The spectral change obtained with denatured DNA was a composite of 2 qualitatively different changes, the change being due solely to the reaction between glyoxal and guanosine residues and the hyperchromic shift of the 260-m[mu] band, which may be a secondary effect of the reaction. From the analysis of these 2 effects, the degree of reaction of the guanosine residues in denatured DNA was estimated to be 72 [degree]/o.