Evidence for circulating bone marrow-derived endothelial cells
Evidence for circulating bone marrow-derived endothelial cells
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DOI：
10.1182/blood.v92.2.362.414k38_362_367
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发表时间：
1998-07-15
期刊：
影响因子：
20.3
通讯作者：
Hammond, WP
中科院分区：
文献类型：
--
作者：
Shi, Q;Rafii, S;Hammond, WP
已经提出造血细胞和内皮细胞来源于一种共同的细胞，即成血管细胞。在这项研究中，我们证明了一个亚群的CD 34（+）细胞有能力分化为内皮细胞在体外存在的碱性成纤维细胞生长因子，胰岛素样生长因子-1，血管内皮生长因子。这些分化的内皮细胞是CD 34+，血管性血友病因子（VWF）染色，并纳入乙酰化低密度脂蛋白（LDL）。这表明可能存在骨髓来源的前体内皮细胞。为了在体内证明这种现象，我们使用了犬骨髓移植模型，其中来自供体和受体的骨髓细胞在遗传上是不同的。移植后6 - 8个月，在降胸主动脉中植入涤纶移植物，使其不渗透以防止周围移植物周围组织的毛细血管向内生长。12周后，取出移植物，通过银染色鉴定具有内皮形态的细胞。使用二（CA）和四核苷酸（GAAA）（n）重复多态性来区分供体和受体DNA，我们观察到只有供体等位基因在来自不可渗透的涤纶移植物上的阳性染色细胞的DNA中检测到。这些结果有力地表明，骨髓中的CD 34（+）细胞亚群可以动员到外周循环中，并可以定植在人造血管的内皮流动表面。(C)1998年，美国血液学会。
It has been proposed that hematopoietic and endothelial cells are derived from a common cell, the hemangioblast. In this study, we demonstrate that a subset of CD34(+) cells have the capacity to differentiate into endothelial cells in vitro in the presence of basic fibroblast growth factor, insulin-like growth factor-1, and vascular endothelial growth factor. These differentiated endothelial cells are CD34+, stain for von Willebrand factor (VWF), and incorporate acetylated low-density lipoprotein (LDL). This suggests the possible existence of a bone marrow-derived precursor endothelial cell. To demonstrate this phenomenon in vivo, we used a canine bone marrow transplantation model, in which the marrow cells from the donor and recipient are genetically distinct. Between 6 to 8 months after transplantation, a Dacron graft, made impervious to prevent capillary ingrowth from the surrounding perigraft tissue, was implanted in the descending thoracic aorta. After 12 weeks, the graft was retrieved, and cells with endothelial morphology were identified by silver nitrate staining. Using the di(CA), and tetranucleotide (GAAA)(n) repeat polymorphisms to distinguish between the donor and recipient DNA, we observed that only donor alleles were detected in DNA from positively stained cells on the impervious Dacron graft. These results strongly suggest that a subset of CD34(+) cells localized in the bone marrow can he mobilized to the peripheral circulation and can colonize endothelial flow surfaces of vascular prostheses. (C) 1998 by The American Society of Hematology.