Characterization of monoclonal antibody against syringate derivatives: application of immunochemical detection of methyl syringate in honey
Characterization of monoclonal antibody against syringate derivatives: application of immunochemical detection of methyl syringate in honey
复制标题
抗丁香酸衍生物单克隆抗体的表征：免疫化学检测蜂蜜中丁香酸甲酯的应用
DOI：
10.1021/acs.jafc.6b01328
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
and Hirohito Ishikawa
中科院分区：
文献类型：
--
作者：
Yoji Kato;Rie Fujinaka;Maki Juri;Yui Yoshiki;Akari Ishisaka;Noritoshi Kitamoto;Yoko Nitta;and Hirohito Ishikawa
丁香酸是植物源化学物质的重要骨架结构之一。丁香酸的衍生物具有一定的生物功能。本研究制备了抗丁香酸类植物化学物质的单抗，并对其进行了鉴定。获得的抗体可与丁香酸甲酯、丁香酸和益母草碱反应。丁香酸甲酯是一种在麦卢卡蜂蜜、其他蜂蜜品种和植物中发现的特有化合物。用高效液相色谱法对麦卢卡蜂蜜进行分级，并检测其与抗体的反应性。抗体与紫丁香酸甲酯洗脱部分发生反应。用该抗体测定蜂蜜中丁香酸甲酯的含量与用高效液相色谱法测定的结果有良好的线性关系。这些结果表明该抗体适用于植物和食品中丁香酸衍生物的免疫化学检测。
Syringic acid is one of the key skeletal structures of plant-derived chemicals. The derivatives of syringic acid have certain biological functions. In this study, a monoclonal antibody to syringic acid-based phytochemicals was prepared and characterized. The obtained antibody reacted with methyl syringate, syringic acid, and leonurine. Methyl syringate is a characteristic compound found in manuka honey, other honey varieties, and plants. Manuka honey was fractionated using HPLC, and the reactivity of the fractions with the antibody was examined. The antibody reacted with the fraction in which methyl syringate was eluted. The amount of methyl syringate in honeys as estimated by ELISA using the antibody had a good linearity compared with that estimated by HPLC. These results suggest that the antibody is applicable for the immunochemical detection of syringic acid derivatives in plants and foods.