DIFFERENTIAL DISTRIBUTION OF 6 CALCIUM-BINDING PROTEINS IN THE RAT OLFACTORY EPITHELIUM DURING POSTNATAL-DEVELOPMENT AND ADULTHOOD
DIFFERENTIAL DISTRIBUTION OF 6 CALCIUM-BINDING PROTEINS IN THE RAT OLFACTORY EPITHELIUM DURING POSTNATAL-DEVELOPMENT AND ADULTHOOD
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DOI：
10.1002/cne.903540308
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发表时间：
1995-04-10
影响因子：
2.5
通讯作者：
POCHET, R
中科院分区：
文献类型：
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作者：
BASTIANELLI, E;POLANS, AS;POCHET, R
气味对受体细胞的刺激导致钙内流，激活转导途径。钙离子受体，如钙调素，可能在细胞内钙离子的变化和初始电事件背后的电导机制之间起中介作用。CA2+受体也可能参与嗅觉信息的后续处理。因此，对这些分子的鉴定和表征将为涉及钙在嗅觉和其他感觉系统中的复杂信号转导通路提供重要信息。本研究描述了出生后大鼠主要嗅觉上皮中六种钙结合蛋白的分布，以确定何时可以检测到不同的钙受体，以及它们是否分离到不同的层或部分上皮。免疫组织化学显示，CaM、Calretinin、Calbindin-D28k、Neurocalin和Recovery in在嗅觉感受器中表达，而在基底细胞中不表达。S-100免疫反应主要局限于筛板周围的神经胶质细胞。在出生后发育期间(从P1到P20)，CaM、Calretinin、Calbindin-D28k和Neurocalin形成了从鼻腔中央到鼻腔外侧的梯度免疫反应，而Recoverin仅在黏膜近端的零星成熟受体中表达。在P20，每种钙结合蛋白的免疫反应模式与成人的情况相同，表明嗅觉上皮在这个阶段已经成熟。嗅神经纤维束从出生到成年期对Calbindin-D28k和Calretinin(束的内部部分)显示出不同的染色模式。嗅神经(嗅神经束的较大外侧部)在第10天出现钙调素免疫反应。神经纤维束的免疫反应性可能反映了与气味辨别相关的进一步的组织程度。(C)1995年Wiley-Liss公司
Odorant stimulation of receptor cells results in a calcium influx that activates the transduction pathway. Ca2+ accepters, such as calmodulin, may mediate between the change in intracellular calcium and the conductance mechanism underlying the initial electrical event. Ca2+ accepters also may participate in subsequent processing of olfactory information. The identification and characterization of these molecules, therefore, should provide important information about the complex signal transduction pathway involving calcium in olfaction as well as other sensory systems.The present study describes the distribution of six calcium-binding proteins in the rat main olfactory epithelium during postnatal development to determine when different Ca2+ accepters can be detected and whether they segregate into different layers or portions of the epithelium. Calmodulin, calretinin, calbindin-D28k, neurocalcin, and recoverin were detected immunohistochemically in olfactory receptors but not in basal cells. S-100 immunoreactivity was restricted to glial cells primarily around the cribriform plate. During postnatal development (from P1 to P20), calmodulin, calretinin, calbindin-D28k, and neurocalcin formed a gradient of immunoreactivity descending from the central to the lateral areas in the nasal cavity, whereas recoverin was expressed only in sporadic, mature receptors in the proximal region of the mucosa. At P20, the immunoreactivity pattern for each calcium-binding protein was identical to the adult profile, indicating that the olfactory epithelium had reached maturity by this stage. Olfactory nerve fiber bundles displayed a differential staining pattern from P1 until adulthood for calbindin-D28k and calretinin (internal portions of bundles). Differential calmodulin immuno-reactivity of olfactory nerves (large external portions of bundles) appeared at P10. The immunoreactivity of the nerve fiber bundles may reflect a further degree of organization relevant to odor discrimination. (C) 1995 Wiley-Liss, Inc.