Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R
Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R
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DOI：
10.1007/bf00224219
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发表时间：
1990-11
影响因子：
5.4
通讯作者：
Nagendra K. Singh;K. Shepherd;Robert A. McIntosh
中科院分区：
文献类型：
--
作者：
Nagendra K. Singh;K. Shepherd;Robert A. McIntosh
控制小麦对三种锈病抗性的基因，叶锈病（Lr 26）、茎锈病（Sr 31）和条锈病（Yr 9）以及位于黑麦1 R染色体短臂上的ω-secalins（Sec 1）相对于彼此和着丝粒定位。对1BL. 1 RS/1 R杂合体与“中国春”双端着丝粒1BL测交的214粒种子（或由它们衍生的家系）的分析表明，对三种锈病的抗性基因之间没有重组，表明对三种锈病的抗性基因之间存在紧密连锁或可能是一个单一的复合位点。抗锈基因位于Sec 1位点5.4 ± 1.7cM处，Sec 1位点位于着丝粒26.1 ± 4.3cM处，基因顺序为着丝粒-Sec 1-Lr 26/Sr 31/Yr 9-端粒。在第二次测交中，使用仅具有秆锈病抗性（SrR）的不同的1BL.1RS易位，尽管后代中非整倍体的比例非常大（45.8%），但上述基因顺序得到证实。此外，在1 RS上，SrR与端粒异染色质（C带）的图距为16.0 ± 4.8cM。这些结果表明，一个非常小的1 RS染色质片段是需要保持对所有三种小麦锈病的抗性。将黑麦碱基因与小麦-黑麦易位系中的抗锈基因分离是可能的，但很难分离。黑麦碱基因被认为与小麦-黑麦易位系中的面团粘性有关。
The genes controlling resistance to three wheat rusts, viz., leaf rust (Lr26), stem rust (Sr31) and stripe or yellow rust (Yr9), and ω-secalins (Sec1), located on the short arm of rye chromosome 1R, were mapped with respect to each other and the centromere. Analysis of 214 seeds (or families derived from them) from testcrosses between a 1BL.1RS/1R heterozygote and ‘Chinese Spring’ ditelocentric 1BL showed no recombination between the genes for resistance to the three rusts, suggesting very tight linkage or perhaps a single complex locus conferring resistance to the three rusts. The rust resistance genes were located 5.4 ± 1.7 cM from theSec1locus, which in turn was located 26.1 ± 4.3 cM from the centromere; the gene order being centromere —Sec1—Lr26/Sr31/Yr9— telomere. In a second test-cross, using a different 1BL.1RS translocation which had only stem rust resistance (SrR), the above gene order was confirmed despite a very large proportion of aneuploids (45.8%) among the progeny. Furthermore, a map distance of 16.0 ± 4.8 cM was estimated forSrRand the telomeric heterochromatin (C-band) on 1RS. These results suggest that a very small segment of 1RS chromatin is required to maintain resistance to all three wheat rusts. It should be possible but difficult to separate the rust resistance genes from the secalin gene(s), which are thought to contribute to dough stickiness of wheat-rye translocation lines carrying 1RS.