CHEMOTACTIC FACTOR CONCENTRATION GRADIENTS IN CHEMOTAXIS ASSAY SYSTEMS
CHEMOTACTIC FACTOR CONCENTRATION GRADIENTS IN CHEMOTAXIS ASSAY SYSTEMS
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DOI：
10.1016/0022-1759(81)90079-x
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发表时间：
1981-01-01
影响因子：
2.2
通讯作者：
ZIGMOND, SH
中科院分区：
文献类型：
--
作者：
LAUFFENBURGER, DA;ZIGMOND, SH
在大多数趋化性测定中，趋化因子梯度被建立，然后通过其通过某些基质的扩散而被破坏。梯度特性取决于测定系统的几何形状、趋化因子的扩散系数和添加的趋化因子的浓度。求解扩散方程以表征当前使用的 3 种趋化性测定中存在的梯度：微孔、琼脂糖下和视觉测定系统。给出了针对不同测定时间和具有不同扩散系数的趋化因子的解决方案。
In most chemotaxis assays the chemotactic factor gradient is established and later destroyed by its diffusion through some matrix. The gradient characteristics depend upon the geometry of the assay system, the diffusion coefficient of the chemotactic factor and the concentration of the chemotactic factor added. The diffusion equations were solved to characterize the gradients present in 3 chemotaxis assays in current use: the millipore, under-agarose and visual assay systems. The solutions are presented for various assay times and for chemotactic factors with various diffusion coefficients.