EFFECTS OF DESENSITIZATION ON THE RESPONSES OF WI-38 AND S49 CELLS TO HORMONES
EFFECTS OF DESENSITIZATION ON THE RESPONSES OF WI-38 AND S49 CELLS TO HORMONES
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DOI：
10.1016/0303-7207(84)90081-9
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发表时间：
1984-01-01
影响因子：
4.1
通讯作者：
BUTCHER, RW
中科院分区：
文献类型：
--
作者：
BARBER, R;GOKA, TJ;BUTCHER, RW
使用完整细胞的实验来定量激素脱敏对培养的成纤维细胞（WI-38）和淋巴瘤细胞（S49）中cAMP积累的影响。WI-38细胞对前列腺素E和肾上腺素的脱敏以及S49细胞对肾上腺素的脱敏均与ED 50在每种情况下向更高激素浓度的偏移有关。这导致了这样的情况，即在低浓度激素的连续刺激下的细胞表现出比用高浓度激素处理的相同细胞更大的cAMP合成随时间的相对下降。因此，根据这些细胞中cAMP积累来定量脱敏的任何一般尝试都必须考虑测定的激素浓度。
Experiments with intact cells were used to quantitate the effects of hormone desensitization on the cAMP accumulation in cultured fibroblasts (WI-38) and lymphoma cells (S49). Desensitization of WI-38 cells to prostaglandin E, and epinephrine and of S49 cells to epinephrine was associated with a shift in the ED50in each case to higher hormone concentrations. This led to the situation where cells under continuous stimulation by low concentrations of hormone demonstrated a greater relative decline in cAMP synthesis with time than the same cells treated with high hormone concentrations. Therefore, any general attempt to quantitate desensitization in terms of cAMP accumulation in these cells must take the hormone concentration of the assay into account.