Serum metabolomics analysis of the intervention effect of whole grain oats on insulin resistance induced by high-fat diet in rats.
Serum metabolomics analysis of the intervention effect of whole grain oats on insulin resistance induced by high-fat diet in rats.
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DOI：
10.1016/j.foodres.2020.109297
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发表时间：
2020-09
影响因子：
8.1
通讯作者：
Meng Zhao;Hong Zhang;Jinming Wang;Dandan Shan;Qian Xu
中科院分区：
文献类型：
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作者：
Meng Zhao;Hong Zhang;Jinming Wang;Dandan Shan;Qian Xu
采用代谢组学方法研究全麦燕麦对高脂饮食诱导的胰岛素抵抗大鼠模型的干预作用。将60只大鼠随机分为6组，包括对照组、HF组和4个燕麦干预组。对照组和HF组分别给予标准饲料和HF饲料。4个燕麦干预组均饲喂特殊的HF日粮，其中HF日粮中的蔗糖和淀粉分别以25%、50%、75%和100%的全麦燕麦粉替代。喂养8周后，采集各组大鼠血清进行生化检测，采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-Q-TOF/MS）进行代谢组学分析。结果表明，WGO具有调节体重、血糖、血脂和炎症反应的作用，提示WGO对HF饮食诱导的IR具有预防作用，且以50%WGO替代即可。通过血清代谢组学分析，发现6种与WGO干预IR密切相关的差异代谢产物，相关代谢途径涉及能量代谢、氨基酸代谢和脂质代谢。
This study aimed to evaluate the intervention effect of whole grain oats (WGO) on a high-fat (HF) diet induced insulin resistance (IR) rat model by metabolomic approach. Sixty rats were randomly allocated into six groups, including the control group, HF group, and four oat-intervention groups. Rats in the control group and HF group were respectively given the standard diet and HF diet respectively. The four oat-intervention groups were fed with a special HF diet, in which sucrose and starch in the HF diet were replaced by whole grain oats powder in different proportions of 25%, 50%, 75%, and 100% respectively. After 8-week feeding, serum samples of all rats were collected for biochemical detection, and the metabolomics analysis was carried out by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The results showed that the WGO could regulate body weight, blood glucose, blood lipid, and inflammatory response, which indicated that WGO could prevent IR induced by the HF diet, and it was effective enough by 50% WGO replacement. By serum metabolomics analysis, six differential metabolites closely related to WGO intervention on IR were found, and the related metabolic pathways involved energy metabolism, amino acid metabolism, and lipid metabolism.