Characterizing effects of subthalamic nucleus deep brain stimulation on methamphetamine-induced circling behavior in hemi-Parkinsonian rats.
Characterizing effects of subthalamic nucleus deep brain stimulation on methamphetamine-induced circling behavior in hemi-Parkinsonian rats.
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DOI：
10.1109/tnsre.2012.2197761
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发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
Grill WM
中科院分区：
文献类型：
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作者：
So RQ;McConnell GC;August AT;Grill WM
单侧6-羟基多巴胺（6-OHDA）毁损大鼠模型常用于研究丘脑底核（DBS）脑深部电刺激（DBS）治疗帕金森病的效果。然而，DBS参数对该动物模型行为的影响缺乏系统的知识。本研究的目的是表征DBS对甲基苯丙胺诱导的单侧6-OHDA损伤大鼠的转圈的影响。测试的DBS参数包括刺激幅度、刺激频率、甲基苯丙胺剂量、刺激极性和电极的解剖位置。当施加适当的刺激幅度和剂量的甲基苯丙胺时，高频刺激（> 130 Hz），而不是低频刺激（< 10 Hz），在不抑制运动的情况下逆转了同侧旋转的偏差。只有当双极刺激期间使用的至少一个电极位于脑内时，才会产生这种特征频率调谐曲线。当选择最有效的电极接触时，双极刺激和单极刺激之间没有差异，表明单极刺激可以用于未来的实验。甲基苯丙胺诱导的转圈是一种简单、可靠和敏感的行为测试，并具有高通量研究单侧损伤大鼠中DBS效应的潜力。
The unilateral 6-hydroxydopamine (6-OHDA) lesioned rat model is frequently used to study the effects of subthalamic nucleus (STN) deep brain stimulation (DBS) for the treatment of Parkinson’s disease. However, systematic knowledge of the effects of DBS parameters on behavior in this animal model is lacking. The goal of this study was to characterize the effects of DBS on methamphetamine-induced circling in the unilateral 6-OHDA lesioned rat. DBS parameters tested include stimulation amplitude, stimulation frequency, methamphetamine dose, stimulation polarity, and anatomical location of the electrode. When an appropriate stimulation amplitude and dose of methamphetamine were applied, high frequency stimulation (> 130 Hz), but not low frequency stimulation (< 10 Hz), reversed the bias in ipsilateral circling without inhibiting movement. This characteristic frequency tuning profile was only generated when at least one electrode used during bipolar stimulation was located within the STN. No difference was found between bipolar stimulation and monopolar stimulation when the most effective electrode contact was selected, indicating that monopolar stimulation could be used in future experiments. Methamphetamine-induced circling is a simple, reliable, and sensitive behavioral test and holds potential for high-throughput study of the effects of STN DBS in unilaterally lesioned rats.