Phase Diagram of Ternary Cholesterol/Palmitoylsphingomyelin/Palmitoyloleoyl-Phosphatidylcholine Mixtures: Spin-Label EPR Study of Lipid-Raft Formation
Phase Diagram of Ternary Cholesterol/Palmitoylsphingomyelin/Palmitoyloleoyl-Phosphatidylcholine Mixtures: Spin-Label EPR Study of Lipid-Raft Formation
复制标题
DOI：
10.1016/j.bpj.2012.03.043
复制
发表时间：
2012-04-18
影响因子：
3.4
通讯作者：
Marsh, Derek
中科院分区：
文献类型：
--
作者：
Ionova, Irina V.;Livshits, Vsevolod A.;Marsh, Derek
对于胆固醇（chol）、N-棕榈酰鞘磷脂（PSM）和1-棕榈酰-2-油酰磷脂酰胆碱（POPC）的典型脂筏混合物，自旋标记磷脂的电子顺磁共振（EPR）（对结构域大小不敏感）用于确定23 ℃下的三元相图。二元POPC/胆混合物，也没有发现相界PSM含量= 77摩尔% PSM的三元混合物。对于PSM/chol，在10-38 mol % chol范围内出现L-β-L-o共存，并且在4.5和7 mol% POPC下发现进一步的连接线。对于脂质组合物，发现指示流体-流体（L-alpha-L-o）相分离的双组分EPR谱：
For canonical lipid raft mixtures of cholesterol (chol), N-palmitoylsphingomyelin (PSM), and 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), electron paramagnetic resonance (EPR) of spin-labeled phospholipids-which is insensitive to domain size-is used to determine the ternary phase diagram at 23 degrees C. No phase boundaries are found for binary POPC/chol mixtures, nor for ternary mixtures with PSM content = 77 mol % PSM. For PSM/chol, L-beta-L-o coexistence occurs over the range 10-38 mol % chol and further tie lines are found at 4.5 and 7 mol% POPC. Two-component EPR spectra indicative of fluid-fluid (L-alpha-L-o) phase separation are found for lipid compositions: 25%