Three-dimensional localization of fluorescent masses deeply embedded in tissue
Three-dimensional localization of fluorescent masses deeply embedded in tissue
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DOI：
10.1088/0031-9155/47/22/311
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发表时间：
2002-11-21
影响因子：
3.5
通讯作者：
Russo, A
中科院分区：
文献类型：
--
作者：
Eidsath, A;Chernomordik, V;Russo, A
在本文中，我们介绍了使用荧光标记的连续波红外荧光成像原型系统对类组织幻影和离体组织板获得的初步结果。除了实验系统本身的设计外，我们还开发了实验条件下光子迁移的理论模型和相关的三维重建算法，以估计荧光标记物的分布。所开发的算法在荧光二维强度分布分析中的应用表明，它们能够以良好的精度（误差小于或等于10%）重建标记物的位置（包括其深度），无论是脂肪内幻影还是离体组织板。
In this paper we present preliminary results obtained for tissue-like phantoms and ex vivo tissue slabs using a prototype system for CW infrared fluorescence imaging of fluorescent markers. Besides the design of the experimental system itself, we have developed a theoretical model of photon migration under experimental conditions and associated 3D reconstruction algorithms to estimate the distribution of fluorescent markers. Application of the developed algorithms in the analysis of 2D intensity distributions of the fluorescent light demonstrated their ability to reconstruct positions of the markers (including their depths) with good accuracy (error less than or equal to 10%) both for the Intralipid phantoms and ex vivo tissue slabs.