Immunoexpression of SALL4 in Wilms Tumors and Developing Kidney
Immunoexpression of SALL4 in Wilms Tumors and Developing Kidney
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DOI：
10.1007/s12253-011-9364-0
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发表时间：
2011-09-01
影响因子：
2.8
通讯作者：
Rakheja, Dinesh
中科院分区：
文献类型：
--
作者：
Deisch, Jeremy;Raisanen, Jack;Rakheja, Dinesh
SALL4是一种锌指转录因子，在胚胎干细胞的遗传和多能性中起作用，并且在SALL4突变引起肾畸形的肾发育中很重要。由于肾母细胞瘤重演肾胚胎发生，我们假设肾母细胞瘤细胞也可能表达SALL4。我们对肾母细胞瘤、肾源性残余物和胎儿肾皮质的组织微阵列切片进行了SALL4的免疫组织化学。一半（52例中的26例）肾母细胞瘤显示SALL 4免疫反应性，范围从强和弥漫性到局灶性和弱。胚基，上皮，和组合胚基和上皮的免疫反应模式进行了鉴定。无病例显示间质染色。在胎肾中，SALL 4表达仅限于胚基和原始上皮在15周的妊娠。SALL4染色在孕龄后期、非肿瘤性出生后肾脏或肾源性剩余物中未见。我们的研究是第一个证明SALL4在肾母细胞瘤和发育中的胎儿肾脏中的免疫反应性。SALL4染色在肾性残余物中的缺乏，肾母细胞瘤的假定前体，是有趣的，并表明肾母细胞瘤具有多能性的质量，可能是缺乏在肾性残余物。
SALL4 is a zinc finger transcription factor that plays a role in the maintainence and pluripotency of embryonic stem cell and is important in renal development where SALL4 mutations give rise to renal malformations. Because Wilms tumor recapitulates renal embryogenesis, we hypothesized that Wilms tumor cells may also express SALL4. We performed immunohistochemistry for SALL4 on tissue microarray sections of Wilms tumors, nephrogenic rests, and fetal renal cortices. Half (26 out of 52) of the Wilms tumors showed SALL4 immunoreactivity, ranging from strong and diffuse to focal and weak. Blastemal, epithelial, and combined blastemal and epithelial patterns of immunoreactivity were identified. No cases showed stromal staining. In the fetal kidney, SALL4 expression was restricted to the blastema and primitive epithelium at 15 weeks' gestation. SALL4 staining was not seen at later gestational ages, in non-neoplastic postnatal kidneys, or in nephrogenic rests. Our study is the first to demonstrate SALL4 immunoreactivity in Wilms tumors and in developing fetal kidney. The absence of SALL4 staining in nephrogenic rests, the presumed precursors of Wilms tumors, is intriguing and suggests that Wilms tumors have a pluripotency quality that may be lacking in nephrogenic rests.