Growth requirements of human cervical epithelial cells in culture
Growth requirements of human cervical epithelial cells in culture
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人宫颈上皮细胞培养的生长要求
DOI：
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发表时间：
1979
影响因子：
6.4
通讯作者：
E. Parkinson
中科院分区：
文献类型：
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作者：
M. Stanley;E. Parkinson
来自成年妇女子宫颈部分表面的二倍体细胞可以在培养物中从单细胞生长。超微结构上，生长细胞集落显示复层鳞状上皮的特征，并表现出这种上皮在体内的一些分化特征。宫颈上皮细胞HCE的成功连续培养取决于非分裂成纤维细胞饲养层的存在，该饲养层为上皮细胞提供附着和生长因子。致死照射的Wi-38、宫颈成纤维细胞或小鼠成纤维细胞系都能有效促进HCE集落形成和生长。氢化可的松和表皮生长因子增加饲养细胞层存在下的HCE集落大小。在5种菌株中研究了培养寿命，范围为20-38个群体倍增时间，来自老年患者的细胞显示出较短的培养寿命。
Diploid cells derived from the portio surface of the cervix uteri of adult women can be grown in culture, from single cells. Ultrastructurally the colonies of growing cells show the features of stratified squamous epithelium and exhibit some of the differentiated characteristics of this epithelium in vivo. The successful serial cultivation of cervical epithelial cells, HCE, depends upon the presence of a non‐dividing fibroblast feeder layer which provides both attachment and growth factors for epithelial cells. Lethally irradiated Wi‐38, cervical fibroblasts or mouse fibroblast lines are all effective in promoting HCE colony formation and growth. Hydrocortisone and epidermal growth factor increase HCE colony size in the presence of a feeder cell layer. Culture lifetime was studied in five strains and ranged from 20–38 population doublings, with cells from older patients exhibiting a shorter culture life‐time.