A Hybrid Biomonitoring System for Gut-Neuron Communication
A Hybrid Biomonitoring System for Gut-Neuron Communication
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DOI：
10.1109/jmems.2020.3000392
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发表时间：
2020-06
影响因子：
2.7
通讯作者：
A. Chapin;Jin-Gu Han;Ta Ho;J. Herberholz;R. Ghodssi
中科院分区：
文献类型：
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作者：
A. Chapin;Jin-Gu Han;Ta Ho;J. Herberholz;R. Ghodssi
本工作提出了一个集成的电化学和电生理生物监测系统，使沿着肠-脑-轴（GBA）的分子信号的研究。体外肠道细胞培养为研究肠道生理学提供了一个可控的、可访问的平台。同样，离体小龙虾腹部神经索为神经信号的电生理学研究提供了模型。我们的系统第一次整合了这些平台，以研究从肠道到神经系统的信号传导，这在体内会影响大脑。该平台由两个相互连接的模块组成：（I）电化学模块（ECM），模拟用于细胞生长并实现神经递质（5-羟色胺（5-HT））检测的Transwell平台，以及（II）电生理学模块（ECM），托管解剖的小龙虾神经索并允许电极可达性用于评估对5-HT的神经反应。整个系统的整合是通过包含一个灵活的加热器，以保持细胞接近体温（38° C），transwell膜修饰，以改善分子扩散（450倍），同时保持良好的细胞相容性，和精确的流量控制5-HT运输从ECM到血管。这项工作实现了模块特定的环境控制，这将最终使肠道和神经细胞之间的分子信号传导的研究，以促进GBA内的两种组织的实时监测。[2020-0151]
This work presents an integrated electrochemical and electrophysiological biomonitoring system, enabling the study of molecular signaling along the gut-brain-axis (GBA). In vitro gut cell cultures provide a controllable, accessible platform to study gut physiology. Similarly, the ex vivo crayfish abdominal nerve cord provides a model for the electrophysiological study of nerve signaling. For the first time, our system integrates these platforms to enable the study of signaling from gut to nervous system, which in vivo would influence the brain. The platform consists of two interconnected modules: (I) the electrochemistry module (ECM), mimicking a Transwell platform for cell growth and enabling neurotransmitter (serotonin (5-HT)) detection, and (II) the electrophysiology module (EPM), hosting a dissected crayfish nerve cord and allowing electrode accessibility for the assessment of nerve responses to 5-HT. Whole system integration is aided by the inclusion of a flexible heater to maintain cells near body temperature (38° C), transwell membrane modification to improve molecular diffusion (450-fold) while maintaining good cell compatibility, and precise flow-controlled 5-HT transport from ECM to EPM. This work achieves module-specific environmental control, which will ultimately enable the study of molecular signaling between gut and nerve cells to facilitate real-time monitoring of both tissues within the GBA. [2020-0151]