Rapid hyperpolarization of rat skeletal muscle induced by insulin.
Rapid hyperpolarization of rat skeletal muscle induced by insulin.
复制标题
胰岛素诱导大鼠骨骼肌快速超极化。
DOI：
10.1016/0005-2736(81)90098-5
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Rogus,EM
中科院分区：
文献类型：
--
作者：
Zierler,K;Rogus,EM
有人提出，胰岛素引起的靶细胞膜间电位差的增加可能是细胞表面胰岛素受体复合物引起至少一些胰岛素代谢作用的机制。如果胰岛素诱导的超极化是常见效应器反应的转换器，那么它必须先于这些反应。该问题之前尚未得到解决，因此尚未寻求对胰岛素的快速反应。使用了两种方法。在一种方法中，快速更换沐浴液以包含超最大浓度的胰岛素，并进行一系列膜电位 Er 的测量。 1 分钟内胰岛素超极化 9.4 mV。在另一种方法中，将纳升量的高浓度胰岛素溶液从微量移液器中喷射到刺穿的肌肉纤维的表面上。 32 次胰岛素注射中，有 21 次在 1 秒内发生超极化；在 11 次对照注射中没有变化。这是迄今为止报道的对胰岛素最快速的反应，并且与胰岛素诱导的超极化可能转导效应反应的假设一致。
It has been proposed that the increase produced by insulin in electrical potential differences across membranes of target cells may be a mechanism by which the cell surface insulin-receptor complex causes at least some of the metabolic effects of insulin. If insulin-induced hyperpolarization is a transducer of common effector responses it must precede those responses. The problem has not been addressed previously, so that rapid responses to insulin have not been sought. Two methods were used. In one method, the bathing solution was changed rapidly so as to include insulin in supramaximal concentrations, and a series of measurements of membrane potentials,Er, were made. Insulin hyperpolarized by 9.4 mV within 1 min. In the other method, nanoliter amounts of highly concentrated insulin solution were ejected from a micropipette onto the surface of an impaled muscle fiber. In 21 out of 32 insulin injections, hyperpolarization occurred within 1 s; in 11 control injections there was no change. This is the most rapid response to insulin yet reported, and is consistent with the hypothesis that insulin-induced hyperpolarization may transduce effector responses.