Cytophaga hutchinsonii SprA and SprT Are Essential Components of the Type IX Secretion System Required for Ca(2+) Acquisition, Cellulose Degradation, and Cell Motility.
Cytophaga hutchinsonii SprA and SprT Are Essential Components of the Type IX Secretion System Required for Ca(2+) Acquisition, Cellulose Degradation, and Cell Motility.
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Cytophaga hutchinsonii SprA 和 SprT 是 Ca2 获取、纤维素降解和细胞运动所需的 IX 型分泌系统的重要组成部分
DOI：
10.3389/fmicb.2021.628555
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发表时间：
2021
影响因子：
5.2
通讯作者：
Lu X
中科院分区：
文献类型：
--
作者：
Gao L;Tan Y;Zhang W;Qi Q;Lu X
IX型分泌系统（T9 SS）是一种新的蛋白质分泌系统，发现于拟杆菌门（Bacteroidetes）。T9 SS参与将毒力因子、细胞表面粘附素和复杂生物聚合物降解酶分泌到细胞表面或细胞外介质。Cytophaga hutchinsonii是一种分布广泛的细菌，它能够有效地消化纤维素并沿固体表面快速沿着。C. hutchinsonii具有T9 SS组分的全套直系同源物。然而，大多数同源蛋白的功能尚未得到证实。In C. hutchinsonii、CHU_0029和CHU_2709在序列上分别与约氏黄杆菌T9 SS组分SprA和SprT相似。本研究采用添加Ca ~（2+）和Mg ~（2+）的复合培养基，获得了单缺失突变体chu_0029（sprA）和chu_2709（sprT）。单一缺失sprA或sprT导致纤维素利用和滑行运动的缺陷。此外，ΔsprA和ΔsprT突变体在缺乏Ca 2+和Mg 2+的培养基中表现出生长缺陷。ICP-MS检测结果表明，ΔsprA和ΔsprT突变体的全细胞和胞内Ca 2+浓度均显著降低，表明SprA和SprT在微量Ca 2+的同化过程中均起重要作用。而ΔsprA和ΔsprT突变体对Mg ~（2+）的同化没有明显影响。通过对野生型和突变体细胞表面蛋白的蛋白质组学分析，我们发现ΔsprA和ΔsprT突变体在大部分T9 SS底物的分泌上存在缺陷。总之，这些结果表明SprA和SprT都是C. hutchinsonii T9 SS是C.哈钦森氏菌本研究进一步阐明了SprA和SprT在T9 SS中的作用，并进一步证实了T9 SS与Ca ~（2+）摄取系统之间的联系。
The type IX secretion system (T9SS) is a novel protein secretion system, which is found in and confined to the phylum Bacteroidetes. T9SS is involved in the secretion of virulence factors, cell surface adhesins, and complex biopolymer degrading enzymes to the cell surface or extracellular medium. Cytophaga hutchinsonii is a widely distributed bacterium, which is able to efficiently digest cellulose and rapidly glide along the solid surfaces. C. hutchinsonii has a full set of orthologs of T9SS components. However, the functions of most homologous proteins have not been verified. In C. hutchinsonii, CHU_0029 and CHU_2709 are similar in sequence to Flavobacterium johnsoniae T9SS components SprA and SprT, respectively. In this study, the single deletion mutants of chu_0029 (sprA) and chu_2709 (sprT) were obtained using a complex medium with the addition of Ca2+ and Mg2+. Single deletion of sprA or sprT resulted in defects in cellulose utilization and gliding motility. Moreover, the ΔsprA and ΔsprT mutants showed growth defects in Ca2+- and Mg2+-deficient media. The results of ICP-MS test showed that both the whole cell and intracellular concentrations of Ca2+ were dramatically reduced in the ΔsprA and ΔsprT mutants, indicating that SprA and SprT are both important for the assimilation of trace amount of Ca2+. While the assimilation of Mg2+ was not obviously influenced in the ΔsprA and ΔsprT mutants. Through proteomics analysis of the cell surface proteins of the wild type and mutants, we found that the ΔsprA and ΔsprT mutants were defective in secretion of the majority of T9SS substrates. Together, these results indicate that SprA and SprT are both essential components of C. hutchinsonii T9SS, which is required for protein secretion, Ca2+ acquisition, cellulose degradation, and gliding motility in C. hutchinsonii. Our study shed more light on the functions of SprA and SprT in T9SS, and further proved the link between the T9SS and Ca2+ uptake system.
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影响因子：
2.2
作者：
Boehm M;Simson D;Escher U;Schmidt AM;Bereswill S;Tegtmeyer N;Backert S;Heimesaat MM
通讯作者：
Heimesaat MM
假单胞菌 T6SS 效应子招募含有 PQS 的外膜囊泡来获取铁
影响因子：
16.6
作者：
Lin J;Zhang W;Cheng J;Yang X;Zhu K;Wang Y;Wei G;Qian PY;Luo ZQ;Shen X
通讯作者：
Shen X
影响因子：
3.2
作者：
Murata, Takeshi;Tseng, Will;Nikaido, Hiroshi
通讯作者：
Nikaido, Hiroshi
影响因子：
64.8
作者：
Lauber F;Deme JC;Lea SM;Berks BC
通讯作者：
Berks BC
影响因子：
1.2
作者：
Davis, Michael R.;Goldberg, Joanna B.
通讯作者：
Goldberg, Joanna B.