Expression of TRPV6 and CaBP-D28k in the egg shell gland (uterus) during the oviposition cycle of the laying hen
Expression of TRPV6 and CaBP-D28k in the egg shell gland (uterus) during the oviposition cycle of the laying hen
复制标题
蛋鸡产卵周期中TRPV6和CaBP-D28k在蛋壳腺（子宫）中的表达
DOI：
10.1080/00071668.2013.791385
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发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Dai, J. J.
中科院分区：
文献类型：
--
作者：
Hou, J. F.;Zhou, Z. L.;Deng, Y. F.;Dai, J. J.
1. The aim of this study was to investigate the localisation of the transient receptor potential vanilloid channel type 6 (TRPV6) in egg shell gland (ESG) and examine the dynamic expression of TRPV6 and Calbindin-d28k (CaBP-D28k), as well as the changes in concentration of total calcium (Ca), total inorganic phosphorus (P), alkaline phosphatase (ALP), parathyroid hormone (PTH) and calcitonin (CT) in plasma during the oviposition cycle. 2. The plasma ALP activity was notably increased at 8 h. In addition, plasma CT was highest at 0 h and significantly lower at 8 h. The change of plasma PTH concentration increased slightly post-oviposition and reached a maximum at 16 h. 3. Immunohistochemical analysis indicated that TRPV6 was strongly localised to the apical luminal epithelium of the mucosa. The mRNA levels of TRPV6 and CaBP-D28k in the ESG remained very low from 0 to 4.5 h, but were significantly increased at 16 h. Furthermore, Western blotting analysis showed that the expression of TRPV6 and CaBP-D28k also reached a maximum at 16 h and was different from the concentration of CaBP-D28k. 4. In conclusion, the epithelial Ca2+ channel TRPV6 is strongly expressed in the epithelial cells of the eggshell gland, and the increase of TRPV6 and CaBP-D28k mRNA and protein expression during eggshell formation suggests that active Ca2+ transcellular transport exerts significant effects in delivering active calcium in the ESG.
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影响因子：
3.2
作者：
LIPPIELLO, L;WASSERMAN, RH
通讯作者：
WASSERMAN, RH
影响因子：
4.1
作者：
S. Striem;A. Bar
通讯作者：
S. Striem;A. Bar
DOI：
10.1016/j.cbpa.2007.09.014
发表时间：
2008
期刊：
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
影响因子：
--
作者：
S. L. Parker;L. Lindsay;Jacquie F. Herbert;C. Murphy;M. Thompson
通讯作者：
S. L. Parker;L. Lindsay;Jacquie F. Herbert;C. Murphy;M. Thompson
影响因子：
3.6
作者：
EASTIN, WC;SPAZIANI, E
通讯作者：
SPAZIANI, E
DOI：
10.1016/0305-0491(75)90369-7
发表时间：
1975-05
期刊：
Comparative biochemistry and physiology. B, Comparative biochemistry
影响因子：
--
作者：
J. W. Pike;R. H. Alvarado
通讯作者：
J. W. Pike;R. H. Alvarado