Analysis of a mutated phage T6 receptor protein of Escherichia coli K12
Analysis of a mutated phage T6 receptor protein of Escherichia coli K12
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DOI：
10.1007/bf00259416
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发表时间：
1990-05
期刊：
影响因子：
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通讯作者：
C. Maier;A. Middendorf;E. Bremer
中科院分区：
文献类型：
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作者：
C. Maier;A. Middendorf;E. Bremer
TSX-206等位基因编码一种改变的TSX蛋白TSX-206，不能再作为T6受体发挥作用。TSX-206蛋白在低底物浓度下仍能有效地介导脱氧腺苷的外膜渗透。筛选到一株能同时利用TSX-206和TSX野生型蛋白作为受体的T6噬菌体突变体T6h3.1。TSX-206等位基因的克隆和DNA序列分析表明，该噬菌体抗性表型与TSX蛋白272个残基第254位的Asn对Tyr的替换有关。
Thetsx-206allele encodes an altered Tsx protein, Tsx-206, that can no longer function as the T6 receptor. We show here that this allele also confers resistance to the Tsx-specific phages III, H3, H8, K9, K18 and Oxl but not to colicin K. The Tsx-206 protein still mediates the efficient permeation of deoxyadenosine across the outer membrane at low substrate concentration. A host-range mutant of phage T6, T6h3.1, was isolated which can use both the Tsx-206 and the Tsx wild-type protein as its receptor. Cloning and DNA sequence analysis of thetsx-206allele showed that the phage resistant phenotype was associated with an Asn to Tyr substitution at position 254 of the 272-residue Tsx protein.