Induced sputum levels of IL-33 and soluble ST2 in young asthmatic children
Induced sputum levels of IL-33 and soluble ST2 in young asthmatic children
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DOI：
10.3109/02770903.2013.816317
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发表时间：
2013-10-01
影响因子：
1.9
通讯作者：
Kamel, Hamzaoui
中科院分区：
文献类型：
--
作者：
Hamzaoui, Agnes;Berraies, Anissa;Kamel, Hamzaoui
目的：白细胞介素-33是一种IL-1家族细胞因子，通过其T1/ST 2受体发出信号，并作为炎症的关键调节因子，特别是与哮喘有关的2型反应。本研究的目的是测量可溶性ST 2（sST 2）和IL-33在哮喘儿童中的表达，这取决于疾病的活动性。方法：对37例明确诊断的哮喘患儿（中度20例，轻度17例）进行研究。采用ELISA法检测血清和诱导痰（IS）标本中IL-33和sST 2水平，并与22名年龄和性别匹配的健康对照组进行比较。采用实时定量PCR检测IS中IL-33和TNF-α mRNA的表达。结果：与健康对照组相比，患者IS和血清中的sST 2和IL-33水平显著升高（p = 0.0001）。sST 2和IL 33的增加在中度哮喘患者中比在轻度哮喘患者中更重要。血清和IS IL-33水平之间观察到显著相关性（r = 0.497; p = 0.0018）。哮喘IS组IL-33 mRNA表达水平高于对照组。哮喘患者TNF-α和IL-33 mRNA表达之间存在显著相关性（r = 0.772，p = 0.0001）。结论：在IS中观察到的sST 2和IL-33值与疾病活动性相关。IS中IL-33 mRNA表达升高及其与TNF-α的相关性反映了在年轻哮喘患者肺中观察到的炎症过程。
Objective: Interleukin-33 is an IL-1 family cytokine which signals via its T1/ST2 receptor, and acts as a key regulator of inflammation, notably the type-2 response implicated in asthma. This study aims to measure the expression of soluble ST2 (sST2) and IL-33 in asthmatic children, depending on disease activity. Methods: Thirty-seven children with well-defined asthma (20 moderate and 17 mild asthmatics) were studied. IL-33 and sST2 were measured by ELISA in serum and induced sputum (IS) samples, and compared with 22 age-and sex-matched healthy controls. Real-time quantitative PCR was used to determine IL-33 and TNF-alpha mRNA expression in IS. Results: sST2 and IL-33 levels in IS and serum were significantly higher in patients compared with healthy controls (p = 0.0001). The increase in sST2 and IL33 was significantly more important in moderate cases than in mild asthma. A significant correlation was observed between serum and IS IL-33 levels (r = 0.497; p = 0.0018). Higher levels of IL-33 mRNA were detected in IS from asthmatics than those observed in controls. A significant correlation was found between TNF-alpha and IL-33 mRNA expression in the asthmatic subjects (r = 0.772, p = 0.0001). Conclusions: Values of sST2 and IL-33 observed in IS were found to correlate with disease activity. Elevated IL-33 mRNA expression in IS and its correlation with TNF-alpha reflected the inflammatory process observed in the lung of young asthmatics.