A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions.
A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions.
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DOI：
10.1016/j.chom.2020.09.011
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发表时间：
2020-11-11
影响因子：
30.3
通讯作者：
Waller RF
中科院分区：
文献类型：
--
作者：
Barylyuk K;Koreny L;Ke H;Butterworth S;Crook OM;Lassadi I;Gupta V;Tromer E;Mourier T;Stevens TJ;Breckels LM;Pain A;Lilley KS;Waller RF
顶复门寄生虫导致重大人类疾病和粮食不安全。它们的巨大成功归功于高度专业化的细胞区室和结构。这些适应推动了它们的识别、非破坏性渗透以及对宿主细胞的精心改造，以促进它们的生长、传播和对抗宿主的防御。独特的顶端复合体细胞区室的进化伴随着巨大的蛋白质组新颖性。因此，一半的顶端复合蛋白是独特的且未表征的。在这里，我们同时确定了全球流行的顶复门寄生虫弓形虫中数千种蛋白质的稳态亚细胞位置。这为这些单细胞真核生物及其特化区室提供了前所未有的全面分子定义，这些数据揭示了蛋白质表达和功能的空间组织、对宿主的适应以及这些病原体的潜在进化轨迹。使用 hyperLOPIT，将弓形虫蛋白分配到其细胞位置 识别与宿主相互作用和适应相关的复杂蛋白质组 图谱揭示了顶复门寄生细胞进化的位点和时间顺序 细胞空间组织与调节和生化程序相关 顶复门蛋白质组基本上是这些寄生虫所特有的，并且许多细胞区室是高度专业化的。 Barylyuk 等人使用空间蛋白质组学方法。同时绘制数千种弓形虫蛋白的位置图，解决该病原体在其细胞器、区室和结构背景下的基因组复杂性。
Apicomplexan parasites cause major human disease and food insecurity. They owe their considerable success to highly specialized cell compartments and structures. These adaptations drive their recognition, nondestructive penetration, and elaborate reengineering of the host’s cells to promote their growth, dissemination, and the countering of host defenses. The evolution of unique apicomplexan cellular compartments is concomitant with vast proteomic novelty. Consequently, half of apicomplexan proteins are unique and uncharacterized. Here, we determine the steady-state subcellular location of thousands of proteins simultaneously within the globally prevalent apicomplexan parasite Toxoplasma gondii. This provides unprecedented comprehensive molecular definition of these unicellular eukaryotes and their specialized compartments, and these data reveal the spatial organizations of protein expression and function, adaptation to hosts, and the underlying evolutionary trajectories of these pathogens. Using hyperLOPIT, Toxoplasma proteins were assigned to their cell location Complex proteomes associated with host interaction and adaptation are identified The atlas reveals sites and chronology of cell evolution of apicomplexan parasitism Cell spatial organization corelates with regulatory and biochemical programs Apicomplexan proteomes are substantially specific to these parasites, and many cellular compartments are highly specialized. Using spatial proteomic methods, Barylyuk et al. simultaneously map the locations of thousands of Toxoplasma proteins, resolving the genomic complexity of this pathogen within the context of its cell organelles, compartments, and structures.
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影响因子：
16.6
作者：
Christoforou A;Mulvey CM;Breckels LM;Geladaki A;Hurrell T;Hayward PC;Naake T;Gatto L;Viner R;Martinez Arias A;Lilley KS
通讯作者：
Lilley KS
影响因子：
6.7
作者：
Biddau M;Bouchut A;Major J;Saveria T;Tottey J;Oka O;van-Lith M;Jennings KE;Ovciarikova J;DeRocher A;Striepen B;Waller RF;Parsons M;Sheiner L
通讯作者：
Sheiner L
影响因子：
14.9
作者：
Deutsch EW;Csordas A;Sun Z;Jarnuczak A;Perez-Riverol Y;Ternent T;Campbell DS;Bernal-Llinares M;Okuda S;Kawano S;Moritz RL;Carver JJ;Wang M;Ishihama Y;Bandeira N;Hermjakob H;Vizcaíno JA
通讯作者：
Vizcaíno JA
DOI：
10.1093/bioinformatics/btu013
发表时间：
2014-05-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Gatto L;Breckels LM;Wieczorek S;Burger T;Lilley KS
通讯作者：
Lilley KS
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y