Characterization of transcriptional activation and DNA-binding functions in the hinge region of the vitamin D receptor.
Characterization of transcriptional activation and DNA-binding functions in the hinge region of the vitamin D receptor.
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维生素 D 受体铰链区转录激活和 DNA 结合功能的表征。
DOI：
10.1021/bi0477182
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Gewirth,DanielT
中科院分区：
文献类型：
--
作者：
Shaffer,PaulL;McDonnell,DonaldP;Gewirth,DanielT
The vitamin D receptor (VDR) is a ligand-responsive transcription factor that forms active, heterodimeric complexes with the 9-cisretinoic acid receptor (RXR) on vitamin D response elements (VDREs). Both proteins consist of an N-terminal DNA-binding domain, a C-terminal ligand-binding domain, and an intervening hinge region. The length requirements of the hinge for both transcriptional regulation and DNA binding have not been studied to date for any member of the steroid hormone superfamily. We have generated a series of internal deletion mutants of the VDR hinge and found that deletion of as few as five amino acids from the C-terminus of the hinge significantly reduces transcriptional activation in vivo. Replacing deleted residues in the C-terminus of the hinge with alanines restored activity, indicating that this section of the hinge acts as a sequence-independent spacer. The hinge region of VDR forms a long helix, and the geometric consequences of this structure may explain the requirement of the hinge region for transcriptional activity. Interestingly, all of the deletion mutants, even those that do not activate transcription, bind VDREs with equal and high affinity, indicating that the defect in these mutants is not their ability to bind VDREs. In contrast to VDR, constructs of RXR containing deletions of up to 14 amino acids in the hinge region exhibit near wild-type transcriptional activity. The ability to delete more of the RXR hinge may be related to the additional plasticity required by its role as the common heterodimer partner for nuclear receptors on differing DNA elements.
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影响因子：
158.5
作者：
TAYLOR, HR;WEST, SK;EMMETT, EA
通讯作者：
EMMETT, EA
缺乏阿司匹林使用和预防白内障的证据。
DOI：
10.1001/archopht.1987.01060090087034
发表时间：
1987
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
West,SK;Muñoz,BE;Newland,HS;Emmett,EA;Taylor,HR
通讯作者：
Taylor,HR
影响因子：
4.1
作者：
H. Taylor
通讯作者：
H. Taylor
用于晶状体混浊临床分级的简单系统。
DOI：
--
发表时间：
1988
期刊：
Yan ke xue bao = Eye science
影响因子：
--
作者：
Taylor,HR;West,SK
通讯作者：
West,SK
对糖尿病患者进行的一项基于人群的研究中白内障的患病率。
影响因子：
13.7
作者：
Klein,BE;Klein,R;Moss,SE
通讯作者：
Moss,SE