THE MECHANISM OF HATCHING IN FUNDULUS-HETEROCLITUS - DEVELOPMENT AND PHYSIOLOGY
THE MECHANISM OF HATCHING IN FUNDULUS-HETEROCLITUS - DEVELOPMENT AND PHYSIOLOGY
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DOI：
10.1002/jez.1402170108
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发表时间：
1981-01-01
影响因子：
--
通讯作者：
TAYLOR, MH
中科院分区：
文献类型：
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作者：
DIMICHELE, L;TAYLOR, MH
研究了异斜毛蝽孵化机制的发育和生理学。孵化腺的组织学检查显示，它们在发育第 5 天或第 5 天左右开始分化。到第 7 天，腺体呈现出颗粒状外观，这与绒毛膜酶的首次出现相关。从第7天到第10天，腺体的生长和绒毛膜酶含量的增加呈指数级增长。细胞大小和绒毛膜酶活性在第 10 天达到峰值，并保持恒定直至孵化。测试了几种药物对孵化的影响。肾上腺素和低浓度的 MS-222 [间氨基苯甲酸乙酯甲磺酸酯] 与低 O2 浓度相结合，可提前卵的中位孵化日期 (HD50)。筒箭毒碱、高浓度的 MS-222 和阿托品完全抑制孵化，后脑横断也是如此。假设正常孵化过程中呼吸应激反应与绒毛膜酶释放有关。
The development and physiology of the hatching mechanism were examined in F. heteroclitus. Histological examination of the hatching glands revealed that they became differentiated on or about day 5 of development. By day 7 the glands took on a granular appearance which was correlated with the first appearance of chorionase. From days 7-10, growth of the glands and their increase in chorionase content were exponential. Cell size and chorionase activity peaked on day 10 and remained constant until hatching. The effects of several drugs were tested for their effects on hatching. Epinephrine and low concentrations of MS-222 [ethyl-m-aminobenzoate methanesulfonate], in conjunction with low O2 concentration, advanced the median hatching date of eggs (HD50). Tubocurarine, high concentrations of MS-222 and atropine inhibited hatching completely, as did transection of the hind brain. A respiratory stress response linked to chorionase release during normal hatching was hypothesized.