ANALYSIS OF THE BACILLUS-SUBTILIS BACTERIOPHAGE-SPP1 AND BACTERIOPHAGE-SF6 GENE-1 PRODUCT - A PROTEIN INVOLVED IN THE INITIATION OF HEADFUL PACKAGING
ANALYSIS OF THE BACILLUS-SUBTILIS BACTERIOPHAGE-SPP1 AND BACTERIOPHAGE-SF6 GENE-1 PRODUCT - A PROTEIN INVOLVED IN THE INITIATION OF HEADFUL PACKAGING
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DOI：
10.1006/viro.1994.1415
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发表时间：
1994-08-01
期刊：
影响因子：
3.7
通讯作者：
ALONSO, JC
中科院分区：
文献类型：
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作者：
CHAI, SH;KRUFT, V;ALONSO, JC
相关枯草芽孢杆菌噬菌体SPP1、SF6和rho 15的基因I产物（GIP）对DNA成熟和包装至关重要。克隆了噬菌体SF6或rho - 15来源的基因1的DNA片段并对其进行了测序。SF6和rho 15 G1P（预测分子质量均为16.7 kDa）与SPP1的G1P具有71%的一致性。三种噬菌体的G1P均包含三个保守片段（I、II、III）。在第I段和第II段，预测了螺旋-螺旋-螺旋DNA结合和核苷酸结合基序。纯化了源自SPP1和SF6的G1P。SPP1 G1P蛋白（20.7 kDa），与另一多肽一起从过表达克隆基因的细胞中纯化，分子量约为13 kDa。这个13 kda的多肽是由基因1的翻译起始信号产生的，因此被称为SPP1 G1P*。SPP1和SF6的G1P都能与含有pac的DNA片段特异性结合，而缺少I片段的G1P*则不能。通过SPP1基因I与SF6基因之间的结构域交换，获得了嵌合G1P蛋白。本文的结果表明，G1P DNA结合基序位于第I段，而G1P::G1P相互作用的主要决定因素可能位于第II段。SPP1 G1P的III段和延长的c端部分可以省略。G1P::G2P相互作用区域仍未被表征。(C) 1994学术出版社，Inc.
Gene I product (GIP) of the related Bacillus subtilis bacteriophages SPP1, SF6, and rho 15 is essential for DNA maturation and packaging. A DNA segment containing gene 1 of phage SF6 or rho 15 origin was cloned and sequenced. SF6 and rho 15 G1P (both with predicted molecular mass of 16.7 kDa) share 71% identity with G1P of SPP1. The G1P of all three phages contains three conserved segments (I, II, and III). Within segments I and II helix-turn-helix DNA binding and nucleotide binding motifs were predicted. G1P of both SPP1 and SF6 origin was purified. SPP1 G1P protein (20.7 kDa), purified from cells overexpressing the cloned gene, purifies together with another polypeptide, having a molecular mass of about 13 kDa. The 13-kDa polypeptide results from a translation start signal within gene 1, and hence was termed SPP1 G1P*. G1P of both SPP1 and SF6 binds specifically to a pac-containing DNA fragment, whereas G1P*, which lacks segment I, does not. Chimeric G1P protiens were obtained by domain swapping between gene I of SPP1 and SF6. The results presented here suggest that the G1P DNA binding motif lies in segment I and the major determinant for G1P::G1P interaction might lie in segment II. Segment III and the extended C-terminal part of SPP1 G1P are dispensable. The G1P::G2P interacting region remains uncharacterized. (C) 1994 Academic Press, Inc.