DISTRIBUTION OF METALLOTHIONEIN IN THE HUMAN CENTRAL-NERVOUS-SYSTEM
DISTRIBUTION OF METALLOTHIONEIN IN THE HUMAN CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1002/glia.440080108
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发表时间：
1993-05-01
期刊：
影响因子：
6.2
通讯作者：
TROOST, D
中科院分区：
文献类型：
--
作者：
BLAAUWGEERS, HGT;SMITT, PAES;TROOST, D
用免疫组织化学方法检测了正常人脑和脊髓中金属结合蛋白金属硫蛋白（MT）的分布。对三名死于非神经系统疾病且无组织病理学中枢神经系统改变的患者的石蜡包埋脑组织进行处理。本研究的结果表明，MT很容易在正常人脑的星形胶质细胞亚群中检测到。MT染色在灰质星形胶质细胞上最强烈，这些细胞具有短而粗壮的突起，可能代表原生质星形胶质细胞。使用抗mt和抗胶质纤维酸性蛋白免疫染色，我们可以证明两个星形胶质细胞亚群是相互排斥的。mt在原生质星形胶质细胞中表达的功能意义并不完全清楚。金属解毒只是脑胶质细胞的众多功能之一。脑胶质细胞染色允许星形胶质细胞分型的发现可能在脑胶质细胞研究和人脑外科病理中具有重要意义。
The distribution of metallothionein (MT), a metal-binding protein, was examined immunohistochemically in the normal human brain and spinal cord. Paraffin-embedded brain tissue from three patients who had died from a non-neurological disease and were free of histopathological central nervous system alterations were processed. The results of the present study demonstrate that MT is readily detectable in a subgroup of astrocytes in the normal human brain. MT staining is most intense on grey matter astrocytes that bear short stout processes and which probably represent protoplasmic astrocytes.Using anti-MT and anti-glial fibrillary acidic protein immunostaining, we could demonstrate two subpopulations of astrocytes that were mutually exclusive. The functional significance of MT-expression in protoplasmic astrocytes is not entirely dear. Metal detoxification is only one of the many postulated functions of MT. The finding that staining for MT permits subtyping of astrocytes may be of great importance in glia research and surgical pathology of the human brain.