SEX DETERMINATION IN DIOECIOUS PLANTS MELANDRIUM-ALBUM AND M-RUBRUM USING HIGH-RESOLUTION FLOW-CYTOMETRY
SEX DETERMINATION IN DIOECIOUS PLANTS MELANDRIUM-ALBUM AND M-RUBRUM USING HIGH-RESOLUTION FLOW-CYTOMETRY
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DOI：
10.1002/cyto.990190203
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发表时间：
1995-02-01
期刊：
影响因子：
--
通讯作者：
GOHDE, W
中科院分区：
文献类型：
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作者：
DOLEZEL, J;GOHDE, W
用高分辨流式细胞术分析了雌雄异株植物野百合和红花百合的细胞核DNA含量。对切碎叶组织分离出的细胞核进行分析后，G(0/1)峰的变异系数很低(0.5-0.70%)。在这两个物种中，由于性染色体的异型性，雌性的DNA含量低于雄性。这使得在同一直方图上区分雌核和雄核的峰成为可能，并允许在两个物种中进行性别鉴定。这些结果表明，在其他异性生殖的雌雄异株植物中也有性别诊断的可能性。在植物发育的早期阶段需要快速性别鉴定的任何领域，这里描述的分析都可能是有趣的。(C)1995年Wiley-Liss公司
High-resolution flow cytometry was used to analyze nuclear DNA content in dioecious plants Melandrium album and M. rubrum. Very low coefficients of variation (0.5-0.70%) of G(0/1) peaks were obtained after the analysis of nuclei isolated by chopping leaf tissues. In both species, females have lower DNA content than males due to sex chromosome heteromorphism. This made it possible to discriminate peaks belonging to female and male nuclei on the same histogram and permitted unam-biguous sex identification in both species. The results indicated the possibility of diagnosing sex also in other heterogametic dioecious plants. The assay described here might be interesting in any area where rapid sex identification is required at an early stage of plant development. (C) 1995 Wiley-Liss, Inc.