HIGH-RESOLUTION MOLECULAR DISCRIMINATION BY RNA
HIGH-RESOLUTION MOLECULAR DISCRIMINATION BY RNA
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DOI：
10.1126/science.7510417
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发表时间：
1994-03-11
期刊：
影响因子：
56.9
通讯作者：
POLISKY, B
中科院分区：
文献类型：
--
作者：
JENISON, RD;GILL, SC;POLISKY, B
通过从寡核苷酸文库中选择与支气管扩张剂茶碱具有高亲和力和特异性的RNA种类进行鉴定。一个RNA分子与茶碱结合，解离常数K-d为0.1微米。这个结合亲和力比RNA分子与咖啡因的亲和力大10,000倍，咖啡因与咖啡因的亲和力只与茶碱不同，只是在N-7位有一个甲基。核磁共振分析表明，茶碱结合后，该RNA分子的构象或动力学发生了显着变化。对与茶碱化学相关的化合物的结合研究揭示了观察到的结合特异性所需的结构特征。这些结果证明了RNA分子表现出极高的配基识别和辨别能力。
Species of RNA that bind with high affinity and specificity to the bronchodilator theophylline were identified by selection from an oligonucleotide library. One RNA molecule binds to theophylline with a dissociation constant K-d of 0.1 mu M. This binding affinity is 10,000-fold greater than the RNA molecule's affinity for caffeine, which differs from theophylline only by a methyl group at nitrogen atom N-7. Analysis by nuclear magnetic resonance indicates that this RNA molecule undergoes a significant change in its conformation or dynamics upon theophylline binding. Binding studies of compounds chemically related to theophylline have revealed structural features required for the observed binding specificity. These results demonstrate the ability of RNA molecules to exhibit an extremely high degree of ligand recognition and discrimination.