Bioresorbable electrospun gelatin/polycaprolactone nanofibrous membrane as a barrier to prevent cardiac postoperative adhesion
Bioresorbable electrospun gelatin/polycaprolactone nanofibrous membrane as a barrier to prevent cardiac postoperative adhesion
复制标题
生物可吸收电纺明胶/聚己内酯纳米纤维膜作为防止心脏术后粘连的屏障。
DOI：
10.1016/j.actbio.2018.10.022
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发表时间：
2019-01-01
影响因子：
9.7
通讯作者：
Liu, Jinfen
中科院分区：
文献类型：
--
作者：
Feng, Bei;Wang, Shoubao;Liu, Jinfen
心脏手术后胸骨和心外膜粘连增加了心脏再次手术的风险和复杂性，这对先天性心脏病患者来说是一个重大挑战。生物可吸收膜可作为屏障防止术后粘连。在此，我们通过静电纺丝制备了生物可吸收明胶/聚己内酯（GT/PCL）复合膜。该膜的特征在于在形态，机械性能和生物相容性方面。然后，我们在兔模型中评价了其作为物理屏障预防心脏手术粘连的有效性。我们的研究结果表明，该膜具有纳米纤维结构，并且足够坚固，可以用于外科手术。对兔心脏成纤维细胞的体外研究表明，该膜具有生物相容性并抑制细胞浸润。进一步应用于兔心脏粘连模型，发现该膜逐渐被吸收，并有效地抵抗胸骨和心外膜粘连。有趣的是，手术后6个月，GT/PCL膜被完全吸收，同时宿主细胞向内生长，形成天然屏障。总的来说，这些结果表明，GT/PCL膜可能是一个合适的屏障，以防止胸骨和心外膜粘连，并可能被用作一种新的心包替代心脏surgery.Statement of SignificanceElectrospinning是一种通用的方法来制备纳米纤维膜的组织工程和再生医学应用。然而，具有微/纳米尺度结构和高孔隙率的电纺膜可能是一个很好的候选人作为屏障，以防止术后粘连。在这里，我们制备了一种电纺GT/PCL纳米纤维膜，并将其用作防止胸骨和心外膜粘连的屏障。结果表明，该膜具有足够的机械强度，良好的生物相容性，并有效地抵抗胸骨和心外膜粘连。更重要的是，这种膜是生物可吸收的，允许宿主细胞同时向内生长，形成天然屏障。我们相信，目前将激发更多的研究纳米材料，以防止术后粘连的应用。(C)2018 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Post-cardiac surgical sternal and epicardial adhesions increase the risk and complexity of cardiac re-operative surgeries, which represent a significant challenge for patients with the congenital cardiac disease. Bioresorbable membranes can serve as barriers to prevent postoperative adhesions. Herein, we fabricated a bioresorbable gelatin/polycaprolactone (GT/PCL) composite membrane via electrospinning. The membrane was characterized in terms of morphology, mechanical properties, and biocompatibility. We then evaluated its efficacy as a physical barrier to prevent cardiac operative adhesions in a rabbit model. Our results showed that the membrane had a nanofibrous structure and was sturdy enough to be handled for the surgical procedures. In vitro studies with rabbit cardiac fibroblasts demonstrated that the membrane was biocompatible and inhibited cell infiltration. Further application of the membrane in a rabbit cardiac adhesion model revealed that the membrane was resorbed gradually and effectively resisted the sternal and epicardial adhesions. Interestingly, six months after the operation, the GT/PCL membrane was completely resorbed with simultaneous ingrowth of host cells to form a natural barrier. Collectively, these results indicated that the GT/PCL membrane might be a suitable barrier to prevent sternal and epicardial adhesions and might be utilized as a novel pericardial substitute for cardiac surgery.Statement of SignificanceElectrospinning is a versatile method to prepare nanofibrous membranes for tissue engineering and regenerative medicine applications. However, with the micro-/nano-scale structure and high porosity, the electrospun membrane might be an excellent candidate as a barrier to prevent postoperative adhesion. Here we prepared an electropun GT/PCL nanofibrous membrane and applied it as a barrier to prevent sternal and epicardial adhesions. Our results showed that the membrane had sufficient mechanical strength, good biocompatibility, and effectively resisted the sternal and epicardial adhesions. What's more, the membrane was bioresorbable and allowed simultaneous ingrowth of host cells to form a natural barrier. We believe that the current will inspire more research on nanomaterials to prevent postoperative adhesion applications. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.