Caffeine inhibition of cytokinesis: Dynamics of cell plate formation-deformationin vivo
Caffeine inhibition of cytokinesis: Dynamics of cell plate formation-deformationin vivo
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咖啡因抑制胞质分裂：体内细胞板形成-变形的动力学
DOI：
10.1007/bf01282302
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
P. K. Hepler
中科院分区：
文献类型：
--
作者：
Carol L. Bonsignore;P. K. Hepler
用Nomarski微分干涉相差显微镜观察了咖啡因对紫露草毛细胞板形成的影响。众所周知，咖啡因是细胞板形成的有效抑制剂。对药物处理的细胞的直接检查表明，细胞板总是出现并离心生长，直到几乎完成。到目前为止，药物处理的细胞与对照细胞无法区分。然而，在咖啡因的存在下，板永远不会完成，而是似乎融化了，直到没有可触结构保留下来。即使细胞在咖啡因中培养24小时后，细胞板也总是上升，随后分解。对咖啡因作用时间的研究表明，在细胞板形成开始前的几分钟内，药物有效地到达靶点。通过用HEPES/KCl缓冲液洗脱咖啡因，该效应部分可逆，但是细胞板达到完成所需的时间延长，并且在某些情况下，板似乎破碎。腺苷在逆转咖啡因对细胞板形成的抑制方面也是部分有效的。
The effect of caffeine on cell plate formation inTradescantiastamen hair cells has been studiedin vivousing Nomarski differential interference contrast microscopy. It is well known that caffeine is a potent inhibitor of cell plate formation. Direct examination of drug-treated cells reveals that the cell plate always arises and grows centrifugally until almost complete. Up to this point drug-treated cells are indistinguishable from controls. However in the presence of caffeine the plate never reaches completion but rather appears to melt away until no refractile structure remains. Even after cells have been cultured for 24 hours in caffeine a cell plate always arises only to subsequently break down. Studies on the time of caffeine action show that within minutes before the onset of cell plate formation, the drug efficiently reaches the target site. This effect is partially reversible by washing out the caffeine with HEPES/KCl buffer, however the time required for the cell plate to reach completion is prolonged and in some instances the plate appears to be fragmented. Adenosine is also partially effective in reversing the caffeine inhibition of cell plate formation.
DOI：
--
发表时间：
1978
期刊：
Am J Physiol Rev. 57
影响因子：
--
作者：
Kawamami M;Tanaka H;Ishikawa E;Endo M
通讯作者：
Endo M