Application of fluorescent triazoles to analytical chemistry. II. Fluorescent derivatization of carboxylic acids.
Application of fluorescent triazoles to analytical chemistry. II. Fluorescent derivatization of carboxylic acids.
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荧光三唑在分析化学中的应用。
DOI：
10.1248/cpb.37.831
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发表时间：
1989
影响因子：
1.7
通讯作者：
T. Kitagawa
中科院分区：
文献类型：
--
作者：
S. Narita;T. Kitagawa
合成了2-(对氨甲基苯基)-N，N-二甲基-2H-苯并三唑基-5-胺(1)及其两个相关化合物(2和3)作为高灵敏的羧酸荧光衍生化试剂。这些试剂与2-溴-1-乙基吡啶四氟硼酸盐活化的羧酸在室温下反应生成相应的荧光酰胺。我们研究了这些试剂的苯甲酰胺衍生物的荧光特性和层析行为。化合物1被选择用于高效液相色谱(HPLC)。建立了一种高灵敏、简便的测定羧酸的荧光高效液相色谱方法。羧酸的检出限为15fmol/次。
2-(p-Aminomethylphenyl)-N, N-dimethyl-2H-benzotriazolyl-5-amine (1) and two related compounds (2 and 3) were synthesized as highly sensitive fluorescence derivatization reagents for carboxylic acids. These reagents reacted with carboxylic acids activated with 2-bromo-1-ethylpyridinium tetrafluoroborate to produce the corresponding fluorescent amides at room temperature. We investigated the fluorescence characteristics and the chromatographic behavior of the benzamide derivatives of the reagents. Compound 1 was selected for use in high-performance liquid chromatography (HPLC). A highly sensitive and convenient fluorescence HPLC method was developed for the determination of carboxylic acids. The detection limit for carboxylic acids was 15 fmol per injection.