L-3,4-dihydroxyphenylalanine-collagen modified PDMS surface for controlled cell culture
L-3,4-dihydroxyphenylalanine-collagen modified PDMS surface for controlled cell culture
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用于受控细胞培养的 L-3,4-二羟基苯丙氨酸胶原蛋白修饰的 PDMS 表面
DOI：
10.1039/c2jm00104g
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发表时间：
2012-01-01
影响因子：
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通讯作者：
Feng, Xi-Zeng
中科院分区：
文献类型：
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作者：
Gao, Chun-Ying;Guo, Yuan-Yuan;Feng, Xi-Zeng
对聚二甲基硅氧烷（PDMS）表面进行改性以改善其润湿性和生物相容性已成为其生物应用的热点。我们在这里报告了一个简单的生物相容性的方法，PDMS改性的L-3，4-二羟基苯丙氨酸（L-Dopa），使PDMS表面的变化从疏水到亲水。分子动力学模拟结果表明，改性过程主要是PDMS表面与L-Dopa分子的酚基之间的货车范德华相互作用。暴露的氨基/羧基基团引起L-Dopa改性的PDMS表面的改善的亲水性，这有利于随后的胶原蛋白的固定。与L-Dopa修饰的PDMS、胶原修饰的PDMS和天然PDMS相比，L-Dopa-胶原修饰的PDMS表面表现出更好的293 T细胞粘附、铺展和图案化生长。在L-Dopa-胶原图案化的PDMS表面上生长的293 T细胞的荧光图像也显示出良好的细胞生长和活性行为，这支持了PDMS表面上的L-Dopa-胶原修饰的生物相容性。PDMS表面的润湿性和生物相容性的改善将有利于PDMS在生物传感器、免疫分析、组织工程等方面的应用。
The modification of the poly(dimethylsiloxane) (PDMS) surface for improved wettability and biocompatibility has been a hot topic for its biological applications. We report here a facile biocompatible approach for PDMS modification by L-3,4-dihydroxyphenylalanine (L-Dopa) which renders the change of the PDMS surface from hydrophobic to hydrophilic. Molecular dynamics simulation ascribes the modification process to mainly van der Waals interactions between the PDMS surface and the phenol group of the L-Dopa molecule. The exposed amino/carboxyl groups give rise to the improved hydrophilicity of the L-Dopa modified PDMS surface, which facilitates the subsequent immobilization of collagen. The L-Dopa-collagen modified PDMS surface demonstrates better adhesion, spreading and patterned growth of 293T cells compared with L-Dopa modified PDMS, collagen modified PDMS and native PDMS. The fluorescence images of the 293T cells growing on the L-Dopa-collagen patterned PDMS surface also show good cell growth and activity behaviors which support the biocompatibility of the modification of L-Dopa-collagen on the PDMS surface. The improved wettability and biocompatibility of PDMS surfaces would be beneficial for PDMS applications in biosensors, immunological assays, tissue engineering and so on.