ANTIGENIC STRUCTURE OF CHIMERAS OF TYPE-1 AND TYPE-3 POLIOVIRUSES INVOLVING ANTIGENIC SITE-2, SITE-3 AND SITE-4
ANTIGENIC STRUCTURE OF CHIMERAS OF TYPE-1 AND TYPE-3 POLIOVIRUSES INVOLVING ANTIGENIC SITE-2, SITE-3 AND SITE-4
复制标题
DOI：
10.1099/0022-1317-72-10-2475
复制
发表时间：
1991-10-01
影响因子：
3.8
通讯作者：
ALMOND, JW
中科院分区：
文献类型：
--
作者：
MINOR, PD;FERGUSON, M;ALMOND, JW
已经制备了嵌合脊髓灰质炎病毒，其中对应于抗原位点2、3和4的1型Sabin毒株的区域被3型Sabin毒株的相应区域取代。操纵一个位点或其中的一个组分通常不会影响其他位点的反应，这表明它们形成了独立的结构特征。插入位点表达3型抗原特性的程度可以通过与多克隆或单克隆抗体反应或通过免疫原性来评估。位点2可在感染性病毒上表达，位点3可在加热的非感染性病毒（C抗原）上表达，但不能在天然病毒粒子上表达。结果是一致的，认为由连续序列的氨基酸组成的网站可以在嵌合体上，而更复杂的网站，如网站4或网站3的天然病毒体，是不容易从3型转移到1型。
Chimeric polioviruses have been made in which regions of the type 1 Sabin strain corresponding to antigenic sites 2, 3 and 4 have been replaced by the corresponding regions of the type 3 Sabin strain. Manipulation of one site or a component of it generally did not affect the reactions of the others, suggesting that they form independent structural features. The extent to which the inserted site expressed the antigenic properties of type 3 could be assessed by reaction with polyclonal or monoclonal antibodies, or by immunogenicity. Site 2 could be expressed on infectious virus and site 3 on heated non-infectious virus (C antigen), but not on the native virion. The results are consistent with the view that sites consisting of a continuous sequence of amino acids may be presented on chimeras, whereas more complex sites, such as site 4 or site 3 of the native virion, are transferred less readily from type 3 to type 1.