Transgenic rice with inducible ethylene production exhibits broad-spectrum disease resistance to the fungal pathogens Magnaporthe oryzae and Rhizoctonia solani
Transgenic rice with inducible ethylene production exhibits broad-spectrum disease resistance to the fungal pathogens Magnaporthe oryzae and Rhizoctonia solani
复制标题
DOI：
10.1111/pbi.12004
复制
发表时间：
2013-01-01
影响因子：
13.8
通讯作者：
Yang, Yinong
中科院分区：
文献类型：
--
作者：
Helliwell, Emily E.;Wang, Qin;Yang, Yinong
稻瘟病（Magnaporthe oryzae）和纹枯病（Rhizoctonia solani）是水稻（Oryza sativa）最具破坏性的两种病害，严重影响作物产量和粮食品质。最近的证据表明，乙烯（ET）可能比水杨酸和茉莉酸在水稻抗病性中发挥更突出的作用。在这项研究中，我们试图通过基因调控水稻内源ET水平来增强对稻瘟病和纹枯病的抗性。在一个强病原体诱导启动子的控制下，通过表达水稻1-氨基环丙烷-1-羧酸合成酶（1-氨基环丙烷-1-羧酸合成酶）基因，获得了诱导产生ET的转基因系。与野生型植物相比，OsACS2过表达系的OsACS2转录本、内源ET和防御基因表达水平显著增加，尤其是在对病原体感染的反应中。更重要的是，转基因株系对一种田间分离的茄蚜和不同种的M.oryzae表现出更强的抗性。对野生型和转基因系的生长速度、世代时间和种子产量的评估显示，野生型和转基因系之间几乎没有差异。这些结果表明，在转基因水稻中病原诱导的ET生产可以增强对坏死性和半营养性真菌病原体的抗性，而不会对作物产量产生负面影响。
Rice blast (Magnaporthe oryzae) and sheath blight (Rhizoctonia solani) are the two most devastating diseases of rice (Oryza sativa), and have severe impacts on crop yield and grain quality. Recent evidence suggests that ethylene (ET) may play a more prominent role than salicylic acid and jasmonic acid in mediating rice disease resistance. In this study, we attempt to genetically manipulate endogenous ET levels in rice for enhancing resistance to rice blast and sheath blight diseases. Transgenic lines with inducible production of ET were generated by expressing the rice ACS2 (1-aminocyclopropane-1-carboxylic acid synthase, a key enzyme of ET biosynthesis) transgene under control of a strong pathogen-inducible promoter. In comparison with the wild-type plant, the OsACS2-overexpression lines showed significantly increased levels of the OsACS2 transcripts, endogenous ET and defence gene expression, especially in response to pathogen infection. More importantly, the transgenic lines exhibited increased resistance to a field isolate of R.solani, as well as different races of M.oryzae. Assessment of the growth rate, generational time and seed production revealed little or no differences between wild type and transgenic lines. These results suggest that pathogen-inducible production of ET in transgenic rice can enhance resistance to necrotrophic and hemibiotrophic fungal pathogens without negatively impacting crop productivity.