Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction
Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction
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DOI：
10.1080/00365510310003265
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发表时间：
2003-01-01
影响因子：
2.1
通讯作者：
Chen, JH
中科院分区：
文献类型：
--
作者：
Chen, X;Yang, XY;Chen, JH
为探讨溶血磷脂酸（LPA）与心肌梗塞的关系，建立了以抗LPA多克隆抗体为基础的斑点免疫金渗滤法（DIFA）测定血清LPA浓度的简便方法，并测定了31例急性心肌梗塞（AMI）患者和12例正常人血清LPA浓度。急性心肌梗死发病后8小时血清LPA水平升高2倍以上。发病后48-72 h血药浓度最高（10.43 mg/L），7 d后仍高于对照组（1.66 mg/L）。AMI患者血清LPA浓度升高提示LPA可能参与AMI相关的心血管系统病理生理过程。本研究建立的简易DIFA法测定血清LPA浓度简便、灵敏度高。
In this study the relation between lysophosphatidic acid (LPA) and myocardial infarction was investigated, the typical and simplified methods for measuring serum LPA concentration by dot immunogold filtration assay (DIFA) based on a polyclonal antibody to LPA were developed, and serum LPA concentrations were measured in 31 patients with acute myocardial infarction (AMI) and 12 controls (blood donors) by DIFA. Serum LPA levels were raised more than twofold 8 h after the onset of AMI. Maximal elevation (10.43 mg/L) was found at 48-72 h following onset and remained higher than the control concentration (1.66 mg/L) 7 days after AMI. The rise in serum LPA concentration in AMI patients suggests that LPA might be involved in AMI-related pathophysiology in the cardiovascular system. The simplified DIFA developed in the present study for measuring serum LPA concentration is convenient and highly sensitive.