Tyrosine phosphorylation of the dense plaque protein paxillin is regulated during smooth muscle contraction.
Tyrosine phosphorylation of the dense plaque protein paxillin is regulated during smooth muscle contraction.
复制标题
致密斑块蛋白桩蛋白的酪氨酸磷酸化在平滑肌收缩过程中受到调节。
DOI：
10.1152/ajpcell.1996.271.5.c1594
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Gunst,SJ
中科院分区：
文献类型：
--
作者：
Wang,Z;Pavalko,FM;Gunst,SJ
调节肌动蛋白丝在膜相关致密斑块（MADP）部位与细胞膜的附着可以使平滑肌细胞调节其细胞结构以响应外部应力的变化。在这项研究中，在MADP蛋白桩蛋白酪氨酸磷酸化的变化进行了测定在收缩和舒张气管平滑肌条的蛋白质免疫印迹。酪氨酸磷酸化桩蛋白增加了3 - 4倍，与力的发展过程中的收缩刺激与乙酰胆碱（ACh），5-羟色胺，KCl和减少冲洗收缩刺激和松弛过程中引起的毛喉素的时间过程相似。免疫沉淀的肌肉提取物与多轮的抗磷酸酪氨酸抗体去除约20%的总桩蛋白在休息的肌肉和约60%的桩蛋白在肌肉中最大限度地刺激乙酰胆碱。这些结果提供了将桩蛋白酪氨酸磷酸化与平滑肌的主动收缩或与体内完全分化组织的任何功能反应相关联的第一个证据。结果与MADP蛋白在平滑肌中调节力发展的作用一致。
Regulation of the attachment of actin filaments to the cell membrane at membrane-associated dense plaque (MADP) sites could allow smooth muscle cells to modulate their cytostructure in response to changes in external stress. In this study, changes in the tyrosine phosphorylation of the MADP protein paxillin were measured by Western blot during the contraction and relaxation of tracheal smooth muscle strips. Tyrosine phosphorylation of paxillin increased by three- to fourfold with a time course similar to force development during contractile stimulation with acetylcholine (ACh), 5-hydroxytryptamine, and KCl and decreased during washout of contractile stimuli and during relaxation induced by forskolin. Immunoprecipitation of muscle extracts with multiple rounds of anti-phosphotyrosine antibody removed approximately 20% of the total paxillin in resting muscles and approximately 60% of paxillin in muscles maximally stimulated with ACh. These results provide the first evidence associating the tyrosine phosphorylation of paxillin with the active contraction of smooth muscle or with any functional response of a fully differentiated tissue in vivo. The results are consistent with a role for MADP proteins in the regulation of force development in smooth muscle.