Epigenetic reprogramming that prevents transgenerational inheritance of the vernalized state.
Epigenetic reprogramming that prevents transgenerational inheritance of the vernalized state.
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DOI：
10.1038/nature13722
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发表时间：
2014-11-27
期刊：
影响因子：
64.8
通讯作者：
Dean, Caroline
中科院分区：
文献类型：
--
作者：
Crevillen, Pedro;Yang, Hongchun;Cui, Xia;Greeff, Christiaan;Trick, Martin;Qiu, Qi;Cao, Xiaofeng;Dean, Caroline
配子和胚胎中表观遗传状态的重编程对于植物和哺乳动物的正确发育至关重要。在植物中，种系起源于花的体细胞组织，需要消除发育过程中或响应外部刺激时在特定位点积累的染色质修饰。如果这种情况发生得效率低下，它可能会导致表观遗传状态从一代遗传到下一代。然而，在大多数情况下，积累的表观遗传修饰在下一代之前被有效地消除。植物中表观遗传重编程的一个重要例子是拟南芥花阻遏物FLC基因座表达的重置。FLC是表观遗传沉默的长期寒冷的过程中所谓的春化。然而，该基因座在种子发育完成之前被重新激活，以确保每一代都需要春化。与我们对春化诱导的Polycomb介导的表观遗传沉默的详细理解相反，对FLC重新激活的机制知之甚少。在这里，我们表明，在jumonji结构域蛋白ELF6的亚型突变受损的FLC在生殖组织中的重新激活，导致遗传的部分春化状态。ELF6具有H3K27me3脱甲基酶活性，并且突变降低了植物中的这种酶活性。与此一致，在下一代中，与野生型相比，FLC基因座处的H3K27me3水平保持更高并且FLC表达保持更低。我们的数据揭示了H3K27去甲基化在植物和哺乳动物胚胎表观遗传状态重编程中的古老作用。
Reprogramming of epigenetic states in gametes and embryos is essential for correct development in plants and mammals. In plants, the germ line arises from somatic tissues of the flower necessitating erasure of chromatin modifications accumulated at specific loci during development or in response to external stimuli. If this occurs inefficiently it can lead to epigenetic states being inherited from one generation to the next. However, in most cases accumulated epigenetic modifications are efficiently erased before the next generation. An important example of epigenetic reprogramming in plants is the resetting of expression of the Arabidopsis thaliana floral repressor FLC locus. FLC is epigenetically silenced by prolonged cold in a process called vernalization. However, the locus is reactivated prior to completion of seed development to ensure a vernalization requirement every generation. In contrast to our detailed understanding of the Polycomb-mediated epigenetic silencing induced by vernalization, little is known about the mechanism involved in the re-activation of FLC. Here we show that a hypomorphic mutation in the jumonji domain protein ELF6 impaired the reactivation of FLC in reproductive tissues, leading to inheritance of a partially vernalized state. ELF6 has H3K27me3 demethylase activity and the mutation reduced this enzymatic activity in planta. Consistent with this, H3K27me3 levels at the FLC locus stayed higher and FLC expression remained lower, than in the wild type in the following generation. Our data reveal an ancient role for H3K27 demethylation in the reprogramming of epigenetic states in plant and mammalian embryos.
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DOI：
10.1126/science.1212959
发表时间：
2011-10-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Schmitz RJ;Schultz MD;Lewsey MG;O'Malley RC;Urich MA;Libiger O;Schork NJ;Ecker JR
通讯作者：
Ecker JR
影响因子：
14.9
作者：
CORPET, F
通讯作者：
CORPET, F
DOI：
10.1126/science.1190614
发表时间：
2010-10-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Feng S;Jacobsen SE;Reik W
通讯作者：
Reik W
影响因子：
7
作者：
Li, Heng;Ruan, Jue;Durbin, Richard
通讯作者：
Durbin, Richard
影响因子：
64.8
作者：
Mosher, Rebecca A.;Melnyk, Charles W.;Baulcombe, David C.
通讯作者：
Baulcombe, David C.