Effect of abscisic acid on heat stress tolerance in the calli from two ecotypes of Phragmites communis
Effect of abscisic acid on heat stress tolerance in the calli from two ecotypes of Phragmites communis
复制标题
DOI：
10.1007/s10535-010-0110-3
复制
发表时间：
2010-12-01
影响因子：
1.5
通讯作者：
Bi, Y.
中科院分区：
文献类型：
--
作者：
Ding, W.;Song, L.;Bi, Y.
沙丘芦苇（DR）和沼泽芦苇（SR）是芦苇（Phragmites communis Trin.）显示出不同的压力耐受性。为了揭示这种差异的分子基础，热胁迫的影响进行了研究，使用愈伤组织来自两个生态型。热胁迫导致两种生态型愈伤组织的离子渗漏增加，生长受到抑制，细胞活力下降，过氧化氢（H2O2）和丙二醛（MDA）含量升高，但DR愈伤组织的耐热性优于SR愈伤组织。在DR愈伤组织中，热胁迫导致内源阿坝含量显著增加，而在SR愈伤组织中则没有。阿坝合成抑制剂氟啶酮的应用加重了热胁迫对DR愈伤组织的伤害，而对SR愈伤组织的影响很小。外源阿坝的施用缓解了两种生态型愈伤组织的热胁迫症状。阿坝处理提高了超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和过氧化物酶的活性，降低了H_2O_2和MDA含量。这些结果表明，高温胁迫下阿坝的合成能力是决定DR比SR耐热性高的一个重要因素。
Dune reed (DR) and swamp reed (SR) are two ecotypes of reed (Phragmites communis Trin.) that displayed differences in stress tolerance. To uncover the molecular basis for such difference, the effects of heat stress were studied using the calli derived from the two ecotypes. Heat stress caused increased ion leakage, inhibited growth, decreased cell viability, and elevated hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents in the calli of both ecotypes, but DR callus showed better heat tolerance than SR callus. In DR callus, heat stress caused significant increase in the endogenous ABA content but not in SR callus. Application of fluridone (an ABA synthesis inhibitor) aggravated the heat stress damages on the DR callus whereas it had only minimal impact on the SR callus. Exogenous application of ABA alleviated the heat stress symptoms in the calli of both ecotypes. ABA treatment increased the activities of superoxide dismutase, catalase, ascorbate peroxidase and peroxidase, and also decreased H2O2 and MDA contents. These results indicate that the ability of ABA synthesis under heat stress is a key factor attributing to the higher heat tolerance of DR than SR.