Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation.
Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation.
复制标题
遗传证据支持酵母 CCR4-NOT 复合物在转录延伸中的作用。
DOI：
10.1093/genetics/158.2.627
复制
发表时间：
2001
期刊：
影响因子：
3.3
通讯作者：
Chen,J
中科院分区：
文献类型：
--
作者：
Denis,CL;Chiang,YC;Cui,Y;Chen,J
The CCR4-NOT complex is involved in the regulation of gene expression both positively and negatively. The repressive effects of the complex appear to result in part from restricting TBP access to noncanonical TATAA binding sites presumably through interaction with multiple TAF proteins. We provide here genetic evidence that the CCR4-NOT complex also plays a role in transcriptional elongation. First, defects in CCR4-NOT components as well as overexpression of theNOT4gene elicited 6-azauracil (6AU) and mycophenolic acid sensitivities, hallmarks of transcriptional elongation defects. A number of other transcription initiation factors known to interact with the CCR4-NOT complex did not elicit these phenotypes nor did defects in factors that reduced mRNA degradation and hence the recycling of NTPs. Second, deletion ofccr4resulted in severe synthetic effects with mutations or deletions in the known elongation factors RPB2, TFIIS, and SPT16. Third, theccr4deletion displayed allele-specific interactions withrpb1alleles that are thought to be important in the control of elongation. Finally, we found that accr4deletion as well as overexpression of theNOT1gene specifically suppressed the cold-sensitive phenotype associated with thespt5-242allele. The only other known suppressors of thisspt5-242allele are factors involved in slowing transcriptional elongation. These genetic results are consistent with the model that the CCR4-NOT complex, in addition to its known effects on initiation, plays a role in aiding the elongation process.
