A transcriptomic comparison of theca and granulosa cells in chicken and cattle follicles reveals ESR2 as a potential regulator of CYP19A1 expression in the theca cells of chicken follicles
A transcriptomic comparison of theca and granulosa cells in chicken and cattle follicles reveals ESR2 as a potential regulator of CYP19A1 expression in the theca cells of chicken follicles
复制标题
鸡和牛卵泡中卵泡膜和颗粒细胞的转录组比较揭示ESR2是鸡卵泡卵泡膜细胞中CYP19A1表达的潜在调节因子
DOI：
10.1016/j.cbd.2018.04.002
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发表时间：
2018-09-01
影响因子：
3
通讯作者：
Gong, Yanzhang
中科院分区：
文献类型：
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作者：
Jing, Ruoxi;Gu, Lantao;Gong, Yanzhang
先前的研究表明，卵泡膜和颗粒细胞层在哺乳动物和鸟类中发挥的作用并不相同，特别是在雌激素的合成方面。这两种细胞类型的功能已在牛中得到很好的表征，但它们在鸡中仍不清楚。为了阐明这一问题，本研究采用加权基因共表达网络分析（WGCNA）对鸡和牛不同卵泡发育阶段的小黄色卵泡（SYF）进行了比较。从WGCNA获得的模块用于进一步鉴定与CYP 19 A1表达相关的关键基因。模块保存分析显示cow_D（LH峰前的卵泡期）和chicken_SYF（直径6 ~ 8 mm的黄色小卵泡）数据集之间具有较高的相似性，每个模块中均鉴定出10个与鸡SYF中CYP 19 A1表达高度相关的顶部枢纽基因。比较鸡SYF和牛卵泡在分化阶段的卵泡膜和颗粒细胞（TC和GC）的转录组，以及上述枢纽基因，揭示了ESR 2是鸡SYF卵泡膜细胞中CYP 19 A1表达的潜在调节因子。此外，通过比较卵泡膜细胞和颗粒细胞的转录组，还鉴定了197个细胞特异性表达基因（179个在卵泡膜细胞中，18个在颗粒细胞中）和235个细胞偏好性表达基因（196个在卵泡膜细胞中，39个在颗粒细胞中）。
Previous studies have shown that theca and granulosa cell layers in follicles do not play the same roles in mammals and birds, especially regarding the synthesis of estrogen. The functions of these two cell types have been well characterized in cattle, but they remain unclear in chickens. To clarify this issue, a comparison of small yellow follicles (SYFs) in chickens and cattle at different follicular development stages was done by weighted gene co-expression network analysis (WGCNA). The modules obtained from WGCNA were used for further identification of the key genes associated with CYP19A1 expression. Module preservation analysis showed high similarity between cow_D (the follicular phase before the LH surge) and chicken_SYF (small yellow follicle between 6 and 8 mm in diameter) datasets, and 10 top hub genes highly associated with CYP19A1 expression in chicken SYFs were identified in each module. A comparison of the transcriptomes of theca and granulosa cells (TCs and GCs) between chicken SYFs and cattle follicles at the differentiation stage, as well as the aforementioned hub genes, revealed that ESR2 is a potential regulator of CYP19A1 expression in the theca cells of chicken SYFs. Furthermore, 197 cell-specific (179 in theca and 18 in granulosa) and 235 cell-biased expressed genes (196 in theca and 39 in granulosa) in chicken small yellow follicles were also identified by transcriptomic comparison of theca and granulosa cells.