Cloning and Expression of a cDNA Encoding an Insoluble Matrix Protein in the Gastroliths of a Crayfish, Procambarus clarkii
Cloning and Expression of a cDNA Encoding an Insoluble Matrix Protein in the Gastroliths of a Crayfish, Procambarus clarkii
复制标题
克氏原螯虾胃石中编码不溶性基质蛋白的 cDNA 的克隆和表达
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
H. Nagasawa
中科院分区：
文献类型：
--
作者：
N. Tsutsui;K. Ishii;Y. Takagi;Toshiki Watanabe;H. Nagasawa
摘要克氏原螯虾的胃石在蜕皮前的胃中形成成对结构，含有碳酸钙和少量有机基质。在这项研究中，编码不溶性基质蛋白的cDNA分离自克氏原杆菌。开放阅读框编码505个氨基酸残基，包括两个独特的重复序列。氨基酸序列的N-末端的一半，其中包括10个氨基酸的重复，表现出高度的相似性，在人类角质形成细胞中合成的蛋白质的外皮蛋白。北方印迹分析显示，编码基质蛋白的mRNA在胃石形成的蜕皮前期胃石盘中特异性表达。在胃石盘，这种mRNA的表达水平相关的胃石伴随着他们的形成的重量增加。器官培养的胃石盘中的mRNA的表达被蜕皮激素，20-羟基蜕皮激素诱导。这些结果进一步证实了基质蛋白与胃石形成的关系。功能分析表明，该蛋白质抑制碳酸钙结晶在溶液系统中，这表明该蛋白质在胃石的钙化中起作用。
Abstract In the crayfish Procambarus clarkii, the gastroliths are formed as a paired structure in the stomach during the premolt period, and contain calcium carbonate and a small amount of an organic matrix. In this investigation, a cDNA encoding an insoluble matrix protein was isolated from P. clarkii. The open reading frame encoded 505 amino acid residues including two unique repeated sequences. The N-terminal half of the amino acid sequence, which included 10-amino-acid repeats, exhibited a high degree of similarity to that of involucrin, a protein synthesized in human keratinocytes. Northern blot analysis revealed that mRNA encoding the matrix protein is specifically expressed in the gastrolith discs during the premolt period in which the gastroliths formed. In the gastrolith discs, levels of expression of this mRNA correlated increases in weights of the gastroliths concomitant with their formation. Organ culture of the gastrolith discs suggested that expression of mRNA in the discs is induced by molting hormone, 20-hydroxyecdysone. These results reinforced the relationship between the matrix protein and formation of the gastroliths. Functional analysis showed that the protein inhibits calcium carbonate crystallization in a solution system, suggesting that the protein plays a role in the calcification of the gastroliths.
DOI：
10.1016/s0021-9258(18)31434-0
发表时间：
1993-06
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Anne George;B. Sabsay;Philip A L Simonian;A. Veis
通讯作者：
Anne George;B. Sabsay;Philip A L Simonian;A. Veis
人外皮蛋白的转谷氨酰胺酶反应性。
DOI：
10.1159/000029905
发表时间：
2000
期刊：
Skin pharmacology and applied skin physiology
影响因子：
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作者：
Lambert,A;Ekambaram,M;Robinson,N;Eckert,RL
通讯作者：
Eckert,RL
影响因子：
2.9
作者：
HAUSCHKA, PV;CARR, SA
通讯作者：
CARR, SA