SarZ is a key regulator of biofilm formation and virulence in Staphylococcus epidermidis.
SarZ is a key regulator of biofilm formation and virulence in Staphylococcus epidermidis.
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DOI：
10.1086/586714
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发表时间：
2008-05
期刊：
影响因子：
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通讯作者：
Li Wang;Min Li;Dandan Dong;T. Bach;D. Sturdevant;C. Vuong;M. Otto;Q. Gao
中科院分区：
文献类型：
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作者：
Li Wang;Min Li;Dandan Dong;T. Bach;D. Sturdevant;C. Vuong;M. Otto;Q. Gao
表皮葡萄球菌（Staphylococcus epidermidis）引起的生物膜相关感染是主要的医院病原体，是公共卫生系统的一个主要问题，但这种重要表型的调控尚不完全清楚。通过对影响生物膜形成的基因进行高度歧视性筛选，基于SarZ对生物膜胞外多糖的生物合成操纵子转录的显著影响，我们确定了转录调节因子SarZ是生物膜形成和生物膜相关感染的一个新的重要决定因素。此外，sarZ还影响了一系列毒力基因的表达，包括影响脂肪酶和蛋白酶表达的基因、对一种重要的人类抗菌肽的抗性以及溶血的基因。我们的研究表明，SarZ调节因子在维持典型的表皮葡萄球菌表型中起关键作用，其特征是明显的生物膜形成和免疫逃避，这可能是表皮葡萄球菌在慢性生物膜相关感染中作为定植生物和病原体成功的原因。
Biofilm-associated infection due to Staphylococcus epidermidis, the leading nosocomial pathogen, is a major problem for the public health system, but the regulation of this important phenotype is not completely understood. Using a highly discriminatory screening procedure for genes that influence biofilm formation, we identified the transcriptional regulator SarZ as a novel important determinant of biofilm formation and biofilm-associated infection, on the basis of the significant impact of sarZ on the transcription of the biosynthetic operon for biofilm exopolysaccharide. In addition, sarZ influenced the expression of a series of virulence genes, including genes that influence the expression of lipases and proteases, resistance to an important human antimicrobial peptide, and hemolysis. Our study indicates that the SarZ regulator has a key role in maintaining the typical S. epidermidis phenotype, which is characterized by pronounced biofilm formation and immune evasion, a likely reason for the success of S. epidermidis as a colonizing organism and pathogen in chronic, biofilm-associated infection.