SHIGA-LIKE TOXIN-PRODUCING ESCHERICHIA-COLI IN SEATTLE CHILDREN - A PROSPECTIVE-STUDY
SHIGA-LIKE TOXIN-PRODUCING ESCHERICHIA-COLI IN SEATTLE CHILDREN - A PROSPECTIVE-STUDY
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DOI：
10.1016/0016-5085(93)91069-t
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发表时间：
1993-12-01
期刊：
影响因子：
29.4
通讯作者：
TARR, PI
中科院分区：
文献类型：
--
作者：
BOKETE, TN;OCALLAHAN, CM;TARR, PI
背景：在美国，粪便中含有志贺样毒素产生0157：H7型大肠埃希氏菌的频率尚不清楚。本研究的目的是确定这些事件发生的频率。方法：在一项为期一年的前瞻性研究中，对西雅图儿童445份粪便中的2225株大肠菌群进行了志贺氏菌毒素I和II的结构基因检测。这些细胞的粘附性和肌动蛋白聚集特性。结果：5例(1.1%)患者有非0157：H7志贺样毒素产生E。大肠埃希菌的分离率高于志贺氏菌和耶尔森氏菌(各0.2%)，但低于弯曲杆菌(2.5%)、E.Coli0157：H7(2.9%)和沙门氏菌(3.4%)。五名患者中只有一人出现了血性腹泻。无一例发生溶血性尿毒症综合征。所有菌株均能在HeLa细胞上局部黏附并诱导肌动蛋白聚集，并产生一种对Vero细胞具有致死性的毒素。在北美儿童人口中，大肠菌群存在于提交细菌培养的粪便中。它们在儿童腹泻中的作用值得更好地定义。
Background:The frequency with which stools contain Shiga-like toxin producingEscherichia colinot belonging to serotype 0157:H7 is unknown in the United States. The aim of this study was to determine the frequency with which theseE. coliare present in stools from children from Seattle submitted for bacteriologic analysis.Methods:2225 coliform colonics from 445 stools submitted for bacterial culture from Seattle children were probed with the structural genes of Shigalike toxins I and II in a 1-year prospective study. The adherence and actin aggregating characteristics of theseE. coliwere subsequently determined.Results:Five (1.1%) patients had non-0157:H7 Shiga-like toxin producingE. coli, a rate of isolation higher thanShigellaorYersinia(0.2% each) but lower thanCampylobacter(2.5%),E. coli0157:H7 (2.9%), orSalmonella(3.4%). Only one of the five patients had bloody diarrhea. None developed hemolytic uremic syndrome. All strains adhered in a localized pattern to, and induced actin aggregation in, HeLa cells, and produced a toxin that was lethal to Vero cells.Conclusions:Non-0157:H7 Shiga-like toxin producingE. coliare present in stools submitted for bacterial culture in a North American childhood population. Their role in childhood diarrhea warrants better definition.